Chicken IgY-based Coproantigen Capture ELISA for Diagnosis of Human Opisthorchiasis

Overview

Journal Parasitol Int

Publisher Elsevier

Specialty Parasitology

Date 2016 May 4

PMID 27140305

Citations 8

Authors

Salma Teimoori

Yuji Arimatsu

Thewarach Laha

Sasithorn Kaewkes

Piya Sereerak

Manop Sripa

Sirikachorn Tangkawattana

Paul J Brindley

Banchob Sripa

Affiliations

Soon will be listed here.

Abstract

Diagnosis of Opisthorchis viverrini infection by conventional stool examination is increasingly difficult due to the low intensity of the infection after several rounds of control programmes in endemic regions as well as coinfections with intestinal flukes. Therefore sensitive and specific diagnostic test is needed. In this study, a coproantigen sandwich ELISA using recombinant O. viverrini cathepsin F (rOv-CF) was developed. This sandwich ELISA employing chicken IgY raised against rOv-CF in combination with rabbit IgG antibody to the somatic O. viverrini antigens showed a lower detection limit (LLD) of 70ng native O. viverrini somatic antigens by spiking the parasite antigens into control feces. When applied to the diagnosis, the IgY-based sandwich ELISA exhibited sensitivity and specificity of 93.3% and 76.7%, respectively, in an investigation of 90 human cases positive or negative for opisthorchiasis. The positive predictive value (PPV) and negative predictive value (NPV) for this coproantigen detection were 66.7% and 95.2%, respectively. This IgY-based sandwich ELISA using parasite cathepsin F detection shows a promising immunodiagnostic alternative for human opisthorchiasis in endemic regions.

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