Evaluation of Rhophilin Associated Tail Protein (ROPN1L) in the Human Liver Fluke Opisthorchis Viverrini for Diagnostic Approach

Overview

Journal Korean J Parasitol

Specialty Parasitology

Date 2020 Sep 2

PMID 32871643

Citations 5

Authors

Amornrat Geadkaew-Krenc

Rudi Grams

Wansika Phadungsil

Wanlapa Chaibangyang

Nanthawat Kosa

Poom Adisakwattana

Paron Dekumyoy

Affiliations

Soon will be listed here.

Abstract

Tegumental and excretory-secretory proteins are reported as diagnostic antigens for human opisthorchiasis. Rhophilin associated tail protein1-like (OvROPN1L) protein of Opisthorchis viverrini sperm tail showed potential as a diagnostic antigen. The OvROPN1L recombinant fragments were assayed for diagnostic antigenicity for human opisthorchiasis using indirect ELISA. The strongest antigenic region was a N-terminus peptide of M1 - P56. One synthetic peptide (P1, L3-Q13) of this region showed the highest antigenicity to opisthorchiasis. Sera from other parasitic infections including Strongyloides stercoralis, hookworm, Taenia spp, minute intestinal flukes, Paragonimus spp showed lower reactivity to P1. Peptide P1 is located in the disordered N-terminus of ROPN1L supporting its suitability as linear epitope. In the Platyhelminthes the N-terminal sequence of ROPN1L is diverging with taxonomic distance further suggesting that peptide P1 has potential as diagnostic tool in the genus Opisthorchis/Clonorchis. It should be further evaluated in combination with peptides derived from other O. viverrini antigens to increase its diagnostic power.

Citing Articles

Investigation of a Serine Protease Inhibitor Active in the Infectious Stage of the Human Liver Fluke .

Salang R, Phadungsil W, Geadkaew-Krenc A, Grams R Pathogens. 2024; 13(8).

PMID: 39204278 PMC: 11356884. DOI: 10.3390/pathogens13080678.

Novel recombinant proteins and peptides from and for liver fluke exposure ELISA.

Mohan S, Natarajan M, Bruno J Biochem Biophys Rep. 2023; 35:101516.

PMID: 37521374 PMC: 10372155. DOI: 10.1016/j.bbrep.2023.101516.

Cystatins from the Human Liver Fluke : Molecular Characterization and Functional Analysis.

Geadkaew-Krenc A, Grams R, Siricoon S, Kosa N, Krenc D, Phadungsil W Pathogens. 2023; 12(7).

PMID: 37513796 PMC: 10386146. DOI: 10.3390/pathogens12070949.

Production and Immunological Characterization of scFv Specific to Epitope of Rhophilin-Associated Tail Protein 1-like (OvROPN1L).

Geadkaew-Krenc A, Krenc D, Thanongsaksrikul J, Grams R, Phadungsil W, Glab-Ampai K Trop Med Infect Dis. 2023; 8(3).

PMID: 36977161 PMC: 10055880. DOI: 10.3390/tropicalmed8030160.

Screening of sperm antigen epitopes by phage display technique and its preliminary clinical application.

Lu J, Ge Y, Xu Y, Tang S, Liang Y Basic Clin Androl. 2022; 32(1):22.

PMID: 36384441 PMC: 9668387. DOI: 10.1186/s12610-022-00172-w.

References

Kelley L, Mezulis S, Yates C, Wass M, Sternberg M . The Phyre2 web portal for protein modeling, prediction and analysis. Nat Protoc. 2015; 10(6):845-58. PMC: 5298202. DOI: 10.1038/nprot.2015.053. View

Sripa B, Bethony J, Sithithaworn P, Kaewkes S, Mairiang E, Loukas A . Opisthorchiasis and Opisthorchis-associated cholangiocarcinoma in Thailand and Laos. Acta Trop. 2010; 120 Suppl 1:S158-68. PMC: 3010517. DOI: 10.1016/j.actatropica.2010.07.006. View

Sato M, Pongvongsa T, Sanguankiat S, Yoonuan T, Dekumyoy P, Kalambaheti T . Copro-DNA diagnosis of Opisthorchis viverrini and Haplorchis taichui infection in an endemic area of Lao PDR. Southeast Asian J Trop Med Public Health. 2010; 41(1):28-35. View

Duenngai K, Boonmars T, Sithithaworn J, Sithithaworn P . Diagnosis of early infection and post chemotherapeutic treatment by copro-DNA detection in experimental opisthorchiasis. Parasitol Res. 2012; 112(1):271-8. DOI: 10.1007/s00436-012-3134-0. View

Drozdetskiy A, Cole C, Procter J, Barton G . JPred4: a protein secondary structure prediction server. Nucleic Acids Res. 2015; 43(W1):W389-94. PMC: 4489285. DOI: 10.1093/nar/gkv332. View

Worasith C, Kamamia C, Yakovleva A, Duenngai K, Wangboon C, Sithithaworn J . Advances in the Diagnosis of Human Opisthorchiasis: Development of Opisthorchis viverrini Antigen Detection in Urine. PLoS Negl Trop Dis. 2015; 9(10):e0004157. PMC: 4618926. DOI: 10.1371/journal.pntd.0004157. View

Rattanachan S, Grams R, Tesana S, Smooker P, Vichasri Grams S . Opisthorchis viverrini: analysis of the sperm-specific rhophilin associated tail protein 1-like. Acta Trop. 2014; 140:34-40. DOI: 10.1016/j.actatropica.2014.08.002. View

Jamornthanyawat N . The diagnosis of human opisthorchiasis. Southeast Asian J Trop Med Public Health. 2003; 33 Suppl 3:86-91. View

Larsen J, Lund O, Nielsen M . Improved method for predicting linear B-cell epitopes. Immunome Res. 2006; 2:2. PMC: 1479323. DOI: 10.1186/1745-7580-2-2. View

10.

Fiedler S, Dudiki T, Vijayaraghavan S, Carr D . Loss of R2D2 proteins ROPN1 and ROPN1L causes defects in murine sperm motility, phosphorylation, and fibrous sheath integrity. Biol Reprod. 2013; 88(2):41. PMC: 4434999. DOI: 10.1095/biolreprod.112.105262. View

11.

Xu K, Qi H . Sperm-specific AKAP3 is a dual-specificity anchoring protein that interacts with both protein kinase a regulatory subunits via conserved N-terminal amphipathic peptides. Mol Reprod Dev. 2014; 81(7):595-607. DOI: 10.1002/mrd.22329. View

12.

Ricciardi A, Ndao M . Diagnosis of parasitic infections: what's going on?. J Biomol Screen. 2014; 20(1):6-21. DOI: 10.1177/1087057114548065. View

13.

Teimoori S, Arimatsu Y, Laha T, Kaewkes S, Sereerak P, Sripa M . Chicken IgY-based coproantigen capture ELISA for diagnosis of human opisthorchiasis. Parasitol Int. 2016; 66(4):443-447. PMC: 5086311. DOI: 10.1016/j.parint.2015.10.011. View

14.

Umesha K, Kumar S, Parvathi A, Duenngai K, Sithithaworn P, Karunasagar I . Opisthorchis viverrini: detection by polymerase chain reaction (PCR) in human stool samples. Exp Parasitol. 2008; 120(4):353-6. DOI: 10.1016/j.exppara.2008.09.004. View

15.

Carr D, Fujita A, Stentz C, Liberty G, Olson G, Narumiya S . Identification of sperm-specific proteins that interact with A-kinase anchoring proteins in a manner similar to the type II regulatory subunit of PKA. J Biol Chem. 2001; 276(20):17332-8. DOI: 10.1074/jbc.M011252200. View