Light-driven Biocatalytic Reduction of α,β-unsaturated Compounds by Ene Reductases Employing Transition Metal Complexes As Photosensitizers

Overview

Journal Catal Sci Technol

Publisher Royal Society of Chemistry

Date 2016 Mar 29

PMID 27019691

Citations 14

Authors

Martyn K Peers

Helen S Toogood

Derren J Heyes

David Mansell

Benjamin J Coe

Nigel S Scrutton

Affiliations

Soon will be listed here.

Abstract

Efficient and cost effective nicotinamide cofactor regeneration is essential for industrial-scale bio-hydrogenations employing flavin-containing biocatalysts such as the Old Yellow Enzymes. A direct flavin regeneration system using visible light to initiate a photoredox cycle and drive biocatalysis is described, and shown to be effective in driving biocatalytic activated alkene reduction. Using Ru(ii) or Ir(iii) complexes as photosensitizers, coupled with an electron transfer mediator (methyl viologen) and sacrificial electron donor (triethanolamine) drives catalytic turnover of two Old Yellow Enzymes with multiple oxidative substrates. Therefore, there is great potential in the development of light-driven biocatalytic systems, providing an alternative to the reliance on enzyme-based cofactor regeneration systems.

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