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Towards Electroenzymatic Processes Involving Old Yellow Enzymes and Mediated Cofactor Regeneration

Overview
Journal Eng Life Sci
Specialty Biomedical Engineering
Date 2020 Jul 7
PMID 32624730
Citations 3
Authors
Andreas Tosstorff
Cora Kroner
Diederik J Opperman
Frank Hollmann
Dirk Holtmann
Affiliations
Soon will be listed here.
Abstract

Old yellow enzymes are able to catalyze asymmetric C=C reductions. A mediated electroenzymatic process to regenerate the NADPH in combination with an old yellow enzyme was investigated. Due to the fact that the overall process was affected by a broad set of parameters, a design of experiments (DoE) approach was chosen to identify suitable process conditions. Process conditions with high productivities of up to 2.27 mM/h in combination with approximately 90% electron transfer efficiency were identified.

Citing Articles

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Hollmann F, Opperman D, Paul C Angew Chem Int Ed Engl. 2020; 60(11):5644-5665.

PMID: 32330347 PMC: 7983917. DOI: 10.1002/anie.202001876.

Metals in Biotechnology: Cr-Driven Stereoselective Reduction of Conjugated C=C Double Bonds.

Rauch M, Gallou Y, Delorme L, Paul C, Arends I, Hollmann F Chembiochem. 2019; 21(8):1112-1115.

PMID: 31713969 PMC: 7217005. DOI: 10.1002/cbic.201900685.

Discovery, Characterisation, Engineering and Applications of Ene Reductases for Industrial Biocatalysis.

Toogood H, Scrutton N ACS Catal. 2019; 8(4):3532-3549.

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