Silencing of the Olfactory Co-receptor Gene in Dendroctonus Armandi Leads to EAG Response Declining to Major Host Volatiles

Overview

Journal Sci Rep

Specialty Science

Date 2016 Mar 17

PMID 26979566

Citations 18

Authors

Ranran Zhang

Guanqun Gao

Hui Chen

Affiliations

Soon will be listed here.

Abstract

In this study, a polymerase chain reaction (PCR) based on homology genes of Orco was utilized to identify DarmOrco, which is essential for olfaction in D. armandi. The results showed that DarmOrco shares significant sequence homology with Orco proteins had known in other insects. Quantitative real-time PCR (qRT-PCR) analysis suggested that DarmOrco was abundantly expressed in adult D. armandi; by contrast, DarmOrco showed trace amounts of expression level in other stages. Of different tissues, DarmOrco expression level was the highest in the antennae. In order to understand the functional significance of Orco, we injected siRNA of DarmOrco into the conjunctivum between the second and third abdominal segments, and evaluated its expression after siRNA injected for 24 h, 48 h and 72 h. The results of qRT-PCR demonstrated that the reduction of mRNA expression level was significant (~80%) in DarmOrco siRNA-treated D. armandi than in water-injected and non-injected controls. The electroantennogram responses of females and males to 11 major volatiles of its host, were also reduced (30~68% for females; 16~70% for males) in siRNA-treated D. armandi compared with the controls. These results suggest that DarmOrco is crucial in mediating odorant perception.

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References

Wu Z, Chen X, Du Y, Zhou J, Zhuge Q . Molecular identification and characterization of the Orco orthologue of Spodoptera litura. Insect Sci. 2013; 20(2):175-82. DOI: 10.1111/j.1744-7917.2011.01483.x. View

Melo A, Rutzler M, Pitts R, Zwiebel L . Identification of a chemosensory receptor from the yellow fever mosquito, Aedes aegypti, that is highly conserved and expressed in olfactory and gustatory organs. Chem Senses. 2004; 29(5):403-10. DOI: 10.1093/chemse/bjh041. View

Nichols A, Chen S, Luetje C . Subunit contributions to insect olfactory receptor function: channel block and odorant recognition. Chem Senses. 2011; 36(9):781-90. PMC: 3195787. DOI: 10.1093/chemse/bjr053. View

Robertson H, Warr C, Carlson J . Molecular evolution of the insect chemoreceptor gene superfamily in Drosophila melanogaster. Proc Natl Acad Sci U S A. 2003; 100 Suppl 2:14537-42. PMC: 304115. DOI: 10.1073/pnas.2335847100. View

Jackson A, Linsley P . Noise amidst the silence: off-target effects of siRNAs?. Trends Genet. 2004; 20(11):521-4. DOI: 10.1016/j.tig.2004.08.006. View

Tanaka K, Uda Y, Ono Y, Nakagawa T, Suwa M, Yamaoka R . Highly selective tuning of a silkworm olfactory receptor to a key mulberry leaf volatile. Curr Biol. 2009; 19(11):881-90. DOI: 10.1016/j.cub.2009.04.035. View

Kumar B, Taylor R, Pask G, Zwiebel L, Newcomb R, Christie D . A conserved aspartic acid is important for agonist (VUAA1) and odorant/tuning receptor-dependent activation of the insect odorant co-receptor (Orco). PLoS One. 2013; 8(7):e70218. PMC: 3720905. DOI: 10.1371/journal.pone.0070218. View

Nakagawa T, Pellegrino M, Sato K, Vosshall L, Touhara K . Amino acid residues contributing to function of the heteromeric insect olfactory receptor complex. PLoS One. 2012; 7(3):e32372. PMC: 3293798. DOI: 10.1371/journal.pone.0032372. View

Zheng W, Zhu C, Peng T, Zhang H . Odorant receptor co-receptor Orco is upregulated by methyl eugenol in male Bactrocera dorsalis (Diptera: Tephritidae). J Insect Physiol. 2012; 58(8):1122-7. DOI: 10.1016/j.jinsphys.2012.05.011. View

10.

Maleszka J, Foret S, Saint R, Maleszka R . RNAi-induced phenotypes suggest a novel role for a chemosensory protein CSP5 in the development of embryonic integument in the honeybee (Apis mellifera). Dev Genes Evol. 2007; 217(3):189-96. DOI: 10.1007/s00427-006-0127-y. View

11.

Jones W, Nguyen T, Kloss B, Lee K, Vosshall L . Functional conservation of an insect odorant receptor gene across 250 million years of evolution. Curr Biol. 2005; 15(4):R119-21. DOI: 10.1016/j.cub.2005.02.007. View

12.

Vosshall L, Hansson B . A unified nomenclature system for the insect olfactory coreceptor. Chem Senses. 2011; 36(6):497-8. DOI: 10.1093/chemse/bjr022. View

13.

Pitts R, Fox A, Zwiebel L . A highly conserved candidate chemoreceptor expressed in both olfactory and gustatory tissues in the malaria vector Anopheles gambiae. Proc Natl Acad Sci U S A. 2004; 101(14):5058-63. PMC: 387373. DOI: 10.1073/pnas.0308146101. View

14.

Livak K, Schmittgen T . Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods. 2002; 25(4):402-8. DOI: 10.1006/meth.2001.1262. View

15.

Sato K, Pellegrino M, Nakagawa T, Nakagawa T, Vosshall L, Touhara K . Insect olfactory receptors are heteromeric ligand-gated ion channels. Nature. 2008; 452(7190):1002-6. DOI: 10.1038/nature06850. View

16.

Pask G, Jones P, Rutzler M, Rinker D, Zwiebel L . Heteromeric Anopheline odorant receptors exhibit distinct channel properties. PLoS One. 2011; 6(12):e28774. PMC: 3235152. DOI: 10.1371/journal.pone.0028774. View

17.

Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S . MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol. 2011; 28(10):2731-9. PMC: 3203626. DOI: 10.1093/molbev/msr121. View

18.

Yang Y, Krieger J, Zhang L, Breer H . The olfactory co-receptor Orco from the migratory locust (Locusta migratoria) and the desert locust (Schistocerca gregaria): identification and expression pattern. Int J Biol Sci. 2012; 8(2):159-70. PMC: 3248701. DOI: 10.7150/ijbs.8.159. View

19.

Zhu F, Xu P, Barbosa R, Choo Y, Leal W . RNAi-based demonstration of direct link between specific odorant receptors and mosquito oviposition behavior. Insect Biochem Mol Biol. 2013; 43(10):916-23. PMC: 3800558. DOI: 10.1016/j.ibmb.2013.07.008. View

20.

Jones P, Pask G, Rinker D, Zwiebel L . Functional agonism of insect odorant receptor ion channels. Proc Natl Acad Sci U S A. 2011; 108(21):8821-5. PMC: 3102409. DOI: 10.1073/pnas.1102425108. View