Production of Antibodies Against Influenza Virus by Somatic Cell Hybrids Between Mouse Myeloma and Primed Spleen Cells

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1977 Jul 1

PMID 268647

Citations 51

Authors

H KOPROWSKI

W GERHARD

C M Croce

Affiliations

Soon will be listed here.

Abstract

We have produced somatic cell hybrids between mouse myeloma cells and spleen cells derived from a BALB/c mouse immunized with purified influenza virus. The hybrid cells were found to produce large amounts of antibodies specific for the hemagglutinin of the virus and were able to induce tumor formation when injected into BALB/c mice.

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References

LITTLEFIELD J . SELECTION OF HYBRIDS FROM MATINGS OF FIBROBLASTS IN VITRO AND THEIR PRESUMED RECOMBINANTS. Science. 1964; 145(3633):709-10. DOI: 10.1126/science.145.3633.709. View

GERHARD W, KOPROWSKI H . Persistence of virus-specific memory B cells in mice CNS. Nature. 1977; 266(5600):360-1. DOI: 10.1038/266360a0. View

GERHARD W . The analysis of the monoclonal immune response to influenza virus. II. The antigenicity of the viral hemagglutinin. J Exp Med. 1976; 144(4):985-95. PMC: 2190443. DOI: 10.1084/jem.144.4.985. View

Kohler G, Milstein C . Derivation of specific antibody-producing tissue culture and tumor lines by cell fusion. Eur J Immunol. 1976; 6(7):511-9. DOI: 10.1002/eji.1830060713. View

Davidson R, GERALD P . Improved techniques for the induction of mammalian cell hybridization by polyethylene glycol. Somatic Cell Genet. 1976; 2(2):165-76. DOI: 10.1007/BF01542629. View

Pontecorvo G . Production of mammalian somatic cell hybrids by means of polyethylene glycol treatment. Somatic Cell Genet. 1975; 1(4):397-400. DOI: 10.1007/BF01538671. View

Rosenthal J, Hayashi K, Notkins A . Comparison of direct and indirect solid-phase microradioimmunoassays for the detection of viral antigens and antiviral antibody. Appl Microbiol. 1973; 25(4):567-73. PMC: 380863. DOI: 10.1128/am.25.4.567-573.1973. View

SEABRIGHT M . A rapid banding technique for human chromosomes. Lancet. 1971; 2(7731):971-2. DOI: 10.1016/s0140-6736(71)90287-x. View

Croce C, Kieba I, KOPROWSKI H . Unidirectional loss of human chromosomes in rat-human hybrids. Exp Cell Res. 1973; 79(2):461-3. DOI: 10.1016/0014-4827(73)90470-9. View

10.

Klinman N, Aschinazi G . The stimulation of splenic foci in vitro. J Immunol. 1971; 106(5):1338-44. View

11.

Coleman M, Dowdle W, Laver W, Schild G, Webster R . Influenzavirus neuraminidase and neuraminidase-inhibition test procedures. Bull World Health Organ. 1973; 48(2):199-202. PMC: 2481006. View

12.

Svasti J, Milstein C . The complete amino acid sequence of a mouse kappa light chain. Biochem J. 1972; 128(2):427-44. PMC: 1173779. DOI: 10.1042/bj1280427. View

13.

Margulies D, Kuehl W, Scharff M . Somatic cell hybridization of mouse myeloma cells. Cell. 1976; 8(3):405-15. DOI: 10.1016/0092-8674(76)90153-7. View

14.

Kohler G, Milstein C . Continuous cultures of fused cells secreting antibody of predefined specificity. Nature. 1975; 256(5517):495-7. DOI: 10.1038/256495a0. View

15.

Fazekas de St Groth , Webster R . Disquisitions of Original Antigenic Sin. I. Evidence in man. J Exp Med. 1966; 124(3):331-45. PMC: 2138235. DOI: 10.1084/jem.124.3.331. View

16.

GERHARD W, Braciale T, Klinman N . The analysis of the monoclonal immune response to influenza virus. I. Production of monoclonal anti-viral antibodies in vitro. Eur J Immunol. 1975; 5(10):720-5. DOI: 10.1002/eji.1830051013. View