Influenzavirus Neuraminidase and Neuraminidase-inhibition Test Procedures

Overview

Journal Bull World Health Organ

Specialty Public Health

Date 1973 Jan 1

PMID 4541685

Citations 158

Authors

M T Coleman

W R Dowdle

W G Laver

G C Schild

R G Webster

Affiliations

Soon will be listed here.

Abstract

The adequate characterization of type A influenzaviruses requires the identification not only of the haemagglutinin but also of the neuraminidase. The neuraminidase-inhibition test, as performed in the two WHO international reference centres for influenza, is described and its use in other laboratories-particularly those collaborating in the WHO programme-is recommended.

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References

Ada G, LIND P . AN IMMUNOLOGICAL STUDY OF AVIAN, VIRAL AND BACTERIAL NEURAMINIDASE BASED ON SPECIFIC INHIBITION OF ENZYME BY ANTIBODY. J Gen Microbiol. 1963; 32:225-33. DOI: 10.1099/00221287-32-2-225. View

Warren L . The thiobarbituric acid assay of sialic acids. J Biol Chem. 1959; 234(8):1971-5. View

Dimmock N . Dependence of the activity of an influenza virus neuraminidase upon Ca2+. J Gen Virol. 1971; 13(3):481-3. DOI: 10.1099/0022-1317-13-3-481. View

Webster R, Laver W . Preparation and properties of antibody directed specifically against the neuraminidase of influenza virus. J Immunol. 1967; 99(1):49-55. View

Aminoff D . Methods for the quantitative estimation of N-acetylneuraminic acid and their application to hydrolysates of sialomucoids. Biochem J. 1961; 81:384-92. PMC: 1243351. DOI: 10.1042/bj0810384. View