Translocation of Immunoglobulin VH Genes in Burkitt Lymphoma

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1982 Sep 1

PMID 6813863

Citations 64

Authors

J Erikson

J Finan

P C Nowell

C M Croce

Affiliations

Soon will be listed here.

Abstract

We have produced cell hybrids between mouse myeloma cells, which do not produce immunoglobulin chains, and Burkitt lymphoma cells (Daudi), which express surface IgM. Daudi Cells carry a reciprocal chromosome translocation between chromosomes 8 and 14, described as (8;14)(q24;q32). The hybrids were studied for the expression of human immunoglobulin chains and human isozyme markers, for the presence of human chromosomes, and for the presence of the human genes for heavy chain variable regions (VH) and mu and gamma chain constant (C) regions. The results indicate that the expressed mu chain gene is on normal chromosome 14 in Daudi cells. We have also determined that the chromosome 14 involved in the translocation (14q+) carries the gene for C mu and C gamma 1-4 and probably several genes for the variable region (V). Certain hybrids had lost both the chromosomes 14 but had retained the abnormal chromosome 8 (8q-) that carries the terminal end of the long arm of chromosome 14. These hybrids were studied for the presence of human VH, C mu,, and C gamma DNA sequences, and the results indicated that the hybrid cells with the 8q- chromosome contained VH genes that not C genes. Therefore, we conclude that, in the Daudi Burkitt lymphoma, the break in chromosome 14 occurred within the chromosome segment containing V region genes. As a result of the translocation some of these VH genes became associated with chromosome 8. It is possible that the expression of malignancy in Burkitt lymphoma is caused by immunoglobulin V region gene translocation resulting in activation of a gene on the long arm of human chromosome 8.

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References

Laemmli U . Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970; 227(5259):680-5. DOI: 10.1038/227680a0. View

SEABRIGHT M . A rapid banding technique for human chromosomes. Lancet. 1971; 2(7731):971-2. DOI: 10.1016/s0140-6736(71)90287-x. View

MANOLOV G, Manolova Y . Marker band in one chromosome 14 from Burkitt lymphomas. Nature. 1972; 237(5349):33-4. DOI: 10.1038/237033a0. View

Bobrow M, Cross J . Differential staining of human and mouse chromosomes in interspecific cell hybrids. Nature. 1974; 251(5470):77-9. DOI: 10.1038/251077a0. View

Southern E . Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975; 98(3):503-17. DOI: 10.1016/s0022-2836(75)80083-0. View

ZECH L, Haglund U, Nilsson K, Klein G . Characteristic chromosomal abnormalities in biopsies and lymphoid-cell lines from patients with Burkitt and non-Burkitt lymphomas. Int J Cancer. 1976; 17(1):47-56. DOI: 10.1002/ijc.2910170108. View

Maniatis T, Kee S, Efstratiadis A, Kafatos F . Amplification and characterization of a beta-globin gene synthesized in vitro. Cell. 1976; 8(2):163-82. DOI: 10.1016/0092-8674(76)90001-5. View

Croce C . Assignment of the integration site for simian virus 40 to chromosome 17 in GM54VA, a human cell line transformed by simian virus 40. Proc Natl Acad Sci U S A. 1977; 74(1):315-8. PMC: 393250. DOI: 10.1073/pnas.74.1.315. View

KOPROWSKI H, GERHARD W, Croce C . Production of antibodies against influenza virus by somatic cell hybrids between mouse myeloma and primed spleen cells. Proc Natl Acad Sci U S A. 1977; 74(7):2985-8. PMC: 431374. DOI: 10.1073/pnas.74.7.2985. View

10.

Ber R, Klein G, Moar M, Povey S, Rosen A, Westman A . Somatic cell hybrids between human lymphoma lines. IV. Establishment and characterization of a P3HR-1/Daudi hybrid. Int J Cancer. 1978; 21(6):707-19. DOI: 10.1002/ijc.2910210607. View

11.

Singer P, Williamson A . A novel interaction involving a polypeptide chain (P33) in covalent linkage with IgM on the surface of a Burkitt lymphoma cell line (Daudi). Eur J Immunol. 1979; 9(3):224-31. DOI: 10.1002/eji.1830090311. View

12.

Miyoshi I, Hiraki S, Kimura I, Miyamoto K, Sato J . 2/8 translocation in a Japanese Burkitt's lymphoma. Experientia. 1979; 35(6):742-3. DOI: 10.1007/BF01968217. View

13.

Croce C, Shander M, Martinis J, Cicurel L, DAncona G, Dolby T . Chromosomal location of the genes for human immunoglobulin heavy chains. Proc Natl Acad Sci U S A. 1979; 76(7):3416-9. PMC: 383836. DOI: 10.1073/pnas.76.7.3416. View

14.

Engel J, Dodgson J . Analysis of the closely linked adult chicken alpha-globin genes in recombinant DNAs. Proc Natl Acad Sci U S A. 1980; 77(5):2596-600. PMC: 349449. DOI: 10.1073/pnas.77.5.2596. View

15.

McCune J, Lingappa V, Fu S, Blobel G, KUNKEL H . Biogenesis of membrane-bound and secreted immunoglobulins. I. Two distinct translation products of human mu-chain, with identical N-termini and different C-termini. J Exp Med. 1980; 152(2):463-8. PMC: 2185946. DOI: 10.1084/jem.152.2.463. View

16.

Dolby T, Devuono J, Croce C . Cloning and partial nucleotide sequence of human immunoglobulin mu chain cDNA from B cells and mouse-human hybridomas. Proc Natl Acad Sci U S A. 1980; 77(10):6027-31. PMC: 350206. DOI: 10.1073/pnas.77.10.6027. View

17.

Matthyssens G, Rabbitts T . Structure and multiplicity of genes for the human immunoglobulin heavy chain variable region. Proc Natl Acad Sci U S A. 1980; 77(11):6561-5. PMC: 350325. DOI: 10.1073/pnas.77.11.6561. View

18.

Shander M, Martinis J, Croce C . Genetics of human immunoglobulins: assignment of the genes for mu, alpha, and gamma immunoglobulin chains to human chromosome 14. Transplant Proc. 1980; 12(3):417-20. View

19.

Bernheim A, Berger R, Lenoir G . Cytogenetic studies on African Burkitt's lymphoma cell lines: t(8;14), t(2;8) and t(8;22) translocations. Cancer Genet Cytogenet. 1981; 3(4):307-15. DOI: 10.1016/0165-4608(81)90039-x. View

20.

Erikson J, Martinis J, Croce C . Assignment of the genes for human lambda immunoglobulin chains to chromosome 22. Nature. 1981; 294(5837):173-5. DOI: 10.1038/294173a0. View