Improved Techniques for the Induction of Mammalian Cell Hybridization by Polyethylene Glycol

Overview

Journal Somatic Cell Genet

Specialty Genetics

Date 1976 Mar 1

PMID 1028164

Citations 142

Authors

R L Davidson

P S GERALD

Affiliations

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Abstract

Modifications in the techniques for the induction of mammalian somatic cell hybridization by polyethylene glycol (PEG) have led to procedures that are rapid, simple, and effective. The basic improvements, for both monolayer and suspension fusions, are a short exposure to PEG and a rapid dilution of PEG following treatment. There is a marked effect of PEG concentration on cell hybridization, and there seem to be inherent differences between cells in terms of the extent of cell fusion induced by PEG.

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