Multiplexed Detection of Influenza A Virus Subtype H5 and H9 Via Quantum Dot-based Immunoassay

Overview

Journal Biosens Bioelectron

Specialties Biochemistry
Biotechnology

Date 2015 Oct 12

PMID 26454828

Citations 27

Authors

Feng Wu

Hang Yuan

Changhua Zhou

Mao Mao

Qian Liu

Huaibin Shen

Yu Cen

Zhifeng Qin

Lan Ma

Ling Song Li

Affiliations

Soon will be listed here.

Abstract

A quantum dot-based lateral flow immunoassay system (QD-LFIAS) was developed to simultaneously detect both influenza A virus subtypes H5 and H9. Water-soluble carboxyl-functionalized quantum dots (QDs) were used as fluorescent tags. The QDs were conjugated to specific influenza A virus subtype H5 and H9 antibodies via an amide bond. When influenza A virus subtype H5 or H9 was added to the QD-LFIAS, the QD-labeled antibodies specifically bound to the H5 or H9 subtype viruses and were then captured by the coating antibodies at test line 1 or 2 to form a sandwich complex. This complex produced a bright fluorescent band in response to 365 nm ultraviolet excitation. The intensity of fluorescence can be detected using an inexpensive, low-maintenance instrument, and the virus concentration directly correlates with the fluorescence intensity. The detection limit of the QD-LFIAS for influenza A virus subtype H5 was 0.016 HAU, and the detection limit of the QD-LFIAS for influenza A virus subtype H9 was 0.25 HAU. The specificity and reproducibility were good. The simple analysis step and objective results that can be obtained within 15 min indicate that this QD-LFIAS is a highly efficient test that can be used to monitor and prevent both Influenza A virus subtypes H5 and H9.

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