MicroRNA Profiles in Hippocampal Granule Cells and Plasma of Rats with Pilocarpine-induced Epilepsy--comparison with Human Epileptic Samples

Overview

Journal Sci Rep

Specialty Science

Date 2015 Sep 19

PMID 26382856

Citations 56

Authors

Paolo Roncon

Marie Soukupova

Anna Binaschi

Chiara Falcicchia

Silvia Zucchini

Manuela Ferracin

Sarah R Langley

Enrico Petretto

Michael R Johnson

Gianluca Marucci

Roberto Michelucci

Guido Rubboli

Michele Simonato

Affiliations

Soon will be listed here.

Abstract

The identification of biomarkers of the transformation of normal to epileptic tissue would help to stratify patients at risk of epilepsy following brain injury, and inform new treatment strategies. MicroRNAs (miRNAs) are an attractive option in this direction. In this study, miRNA microarrays were performed on laser-microdissected hippocampal granule cell layer (GCL) and on plasma, at different time points in the development of pilocarpine-induced epilepsy in the rat: latency, first spontaneous seizure and chronic epileptic phase. Sixty-three miRNAs were differentially expressed in the GCL when considering all time points. Three main clusters were identified that separated the control and chronic phase groups from the latency group and from the first spontaneous seizure group. MiRNAs from rats in the chronic phase were compared to those obtained from the laser-microdissected GCL of epileptic patients, identifying several miRNAs (miR-21-5p, miR-23a-5p, miR-146a-5p and miR-181c-5p) that were up-regulated in both human and rat epileptic tissue. Analysis of plasma samples revealed different levels between control and pilocarpine-treated animals for 27 miRNAs. Two main clusters were identified that segregated controls from all other groups. Those miRNAs that are altered in plasma before the first spontaneous seizure, like miR-9a-3p, may be proposed as putative biomarkers of epileptogenesis.

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