SS-31 Attenuates TNF-α Induced Cytokine Release from C2C12 Myotubes

Overview

Journal Redox Biol

Specialties Biology
Cell Biology
Endocrinology

Date 2015 Aug 21

PMID 26291279

Citations 18

Authors

Adam P Lightfoot

Giorgos K Sakellariou

Gareth A Nye

Francis McArdle

Malcolm J Jackson

Richard D Griffiths

Anne McArdle

Affiliations

Soon will be listed here.

Abstract

TNF-α is a key inflammatory mediator and is proposed to induce transcriptional responses via the mitochondrial generation of Reactive Oxygen Species (ROS). The aim of this study was to determine the effect of TNF-α on the production of myokines by skeletal muscle. Significant increases were seen in the release of IL-6, MCP-1/CCL2, RANTES/CCL5 and KC/CXCL1 and this release was inhibited by treatment with Brefeldin A, suggesting a golgi-mediated release of cytokines by muscle cells. An increase was also seen in superoxide in response to treatment with TNF-α, which was localised to the mitochondria and this was also associated with activation of NF-κB. The changes in superoxide, activation of NF-kB and release of myokines were attenuated following pre-treatment with SS-31 peptide indicating that the ability of TNF-α to induce myokine release may be mediated through mitochondrial superoxide, which is, at least in part, associated with activation of the redox sensitive transcription factor NF-kB.

Citing Articles

Ursolic Acid Alleviates Cancer Cachexia and Prevents Muscle Wasting via Activating SIRT1.

Tao W, Ouyang Z, Liao Z, Li L, Zhang Y, Gao J Cancers (Basel). 2023; 15(8).

PMID: 37190306 PMC: 10136986. DOI: 10.3390/cancers15082378.

Reactive Oxygen and Nitrogen Species (RONS) and Cytokines-Myokines Involved in Glucose Uptake and Insulin Resistance in Skeletal Muscle.

Llanos P, Palomero J Cells. 2022; 11(24).

PMID: 36552772 PMC: 9776436. DOI: 10.3390/cells11244008.

Major histocompatibility complex I-induced endoplasmic reticulum stress mediates the secretion of pro-inflammatory muscle-derived cytokines.

Thoma A, Earl K, Goljanek-Whysall K, Lightfoot A J Cell Mol Med. 2022; 26(24):6032-6041.

PMID: 36426551 PMC: 9753450. DOI: 10.1111/jcmm.17621.

The effect of disease-modifying anti-rheumatic drugs on skeletal muscle mass in rheumatoid arthritis patients: a systematic review with meta-analysis.

Hein T, Peterson L, Bartikoski B, Portes J, Espirito Santo R, Xavier R Arthritis Res Ther. 2022; 24(1):171.

PMID: 35854372 PMC: 9295282. DOI: 10.1186/s13075-022-02858-y.

SS-31 does not prevent or reduce muscle atrophy 7 days after a 65 kdyne contusion spinal cord injury in young male mice.

Graham Z, Deberry J, Cardozo C, Bamman M Physiol Rep. 2022; 10(10):e15266.

PMID: 35611788 PMC: 9131615. DOI: 10.14814/phy2.15266.

References

Li Y, Atkins C, Sweatt J, Reid M . Mitochondria mediate tumor necrosis factor-alpha/NF-kappaB signaling in skeletal muscle myotubes. Antioxid Redox Signal. 2001; 1(1):97-104. DOI: 10.1089/ars.1999.1.1-97. View

Reid M, Moylan J . Beyond atrophy: redox mechanisms of muscle dysfunction in chronic inflammatory disease. J Physiol. 2011; 589(Pt 9):2171-9. PMC: 3098696. DOI: 10.1113/jphysiol.2010.203356. View

Riedemann N, Neff T, Guo R, Bernacki K, Laudes I, Sarma J . Protective effects of IL-6 blockade in sepsis are linked to reduced C5a receptor expression. J Immunol. 2002; 170(1):503-7. DOI: 10.4049/jimmunol.170.1.503. View

Robinson K, Janes M, Pehar M, Monette J, Ross M, Hagen T . Selective fluorescent imaging of superoxide in vivo using ethidium-based probes. Proc Natl Acad Sci U S A. 2006; 103(41):15038-43. PMC: 1586181. DOI: 10.1073/pnas.0601945103. View

Peterson J, Feeback K, Baas J, Pizza F . Tumor necrosis factor-alpha promotes the accumulation of neutrophils and macrophages in skeletal muscle. J Appl Physiol (1985). 2006; 101(5):1394-9. DOI: 10.1152/japplphysiol.01453.2005. View

Lacerda L, Smith R, Opie L, Lecour S . TNFalpha-induced cytoprotection requires the production of free radicals within mitochondria in C2C12 myotubes. Life Sci. 2006; 79(23):2194-201. DOI: 10.1016/j.lfs.2006.07.020. View

Shireman P, Contreras-Shannon V, Ochoa O, Karia B, Michalek J, McManus L . MCP-1 deficiency causes altered inflammation with impaired skeletal muscle regeneration. J Leukoc Biol. 2006; 81(3):775-85. DOI: 10.1189/jlb.0506356. View

Chomczynski P, Sacchi N . The single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction: twenty-something years on. Nat Protoc. 2007; 1(2):581-5. DOI: 10.1038/nprot.2006.83. View

Lang C, Frost R, Vary T . Regulation of muscle protein synthesis during sepsis and inflammation. Am J Physiol Endocrinol Metab. 2007; 293(2):E453-9. DOI: 10.1152/ajpendo.00204.2007. View

10.

Hardin B, Campbell K, Smith J, Arbogast S, Smith J, Moylan J . TNF-alpha acts via TNFR1 and muscle-derived oxidants to depress myofibrillar force in murine skeletal muscle. J Appl Physiol (1985). 2008; 104(3):694-9. DOI: 10.1152/japplphysiol.00898.2007. View

11.

Nielsen S, Pedersen B . Skeletal muscle as an immunogenic organ. Curr Opin Pharmacol. 2008; 8(3):346-51. DOI: 10.1016/j.coph.2008.02.005. View

12.

Pedersen B, Febbraio M . Muscle as an endocrine organ: focus on muscle-derived interleukin-6. Physiol Rev. 2008; 88(4):1379-406. DOI: 10.1152/physrev.90100.2007. View

13.

Scheele C, Nielsen S, Pedersen B . ROS and myokines promote muscle adaptation to exercise. Trends Endocrinol Metab. 2009; 20(3):95-9. DOI: 10.1016/j.tem.2008.12.002. View

14.

Maack C, Bohm M . Targeting mitochondrial oxidative stress in heart failure throttling the afterburner. J Am Coll Cardiol. 2011; 58(1):83-6. DOI: 10.1016/j.jacc.2011.01.032. View

15.

Dai D, Chen T, Szeto H, Nieves-Cintron M, Kutyavin V, Santana L . Mitochondrial targeted antioxidant Peptide ameliorates hypertensive cardiomyopathy. J Am Coll Cardiol. 2011; 58(1):73-82. PMC: 3742010. DOI: 10.1016/j.jacc.2010.12.044. View

16.

Lacy P, Stow J . Cytokine release from innate immune cells: association with diverse membrane trafficking pathways. Blood. 2011; 118(1):9-18. DOI: 10.1182/blood-2010-08-265892. View

17.

Yoon J, Song P, Jang J, Kim D, Choi S, Kim J . Proteomic analysis of tumor necrosis factor-alpha (TNF-α)-induced L6 myotube secretome reveals novel TNF-α-dependent myokines in diabetic skeletal muscle. J Proteome Res. 2011; 10(12):5315-25. DOI: 10.1021/pr200573b. View

18.

Beyer I, Njemini R, Bautmans I, Demanet C, Bergmann P, Mets T . Inflammation-related muscle weakness and fatigue in geriatric patients. Exp Gerontol. 2011; 47(1):52-9. DOI: 10.1016/j.exger.2011.10.005. View

19.

Bostrom P, Wu J, Jedrychowski M, Korde A, Ye L, Lo J . A PGC1-α-dependent myokine that drives brown-fat-like development of white fat and thermogenesis. Nature. 2012; 481(7382):463-8. PMC: 3522098. DOI: 10.1038/nature10777. View

20.

Pedersen B . Muscular interleukin-6 and its role as an energy sensor. Med Sci Sports Exerc. 2011; 44(3):392-6. DOI: 10.1249/MSS.0b013e31822f94ac. View