Amelogenin and Enamel Biomimetics

Overview

Journal J Mater Chem B

Publisher Royal Society of Chemistry

Specialty Biomedical Engineering

Date 2015 Aug 8

PMID 26251723

Citations 62

Authors

Qichao Ruan

Janet Moradian-Oldak

Affiliations

Soon will be listed here.

Abstract

Mature tooth enamel is acellular and does not regenerate itself. Developing technologies that rebuild tooth enamel and preserve tooth structure is therefore of great interest. Considering the importance of amelogenin protein in dental enamel formation, its ability to control apatite mineralization , and its potential to be applied in fabrication of future bio-inspired dental material this review focuses on two major subjects: amelogenin and enamel biomimetics. We review the most recent findings on amelogenin secondary and tertiary structural properties with a focus on its interactions with different targets including other enamel proteins, apatite mineral, and phospholipids. Following a brief overview of enamel hierarchical structure and its mechanical properties we will present the state-of-the-art strategies in the biomimetic reconstruction of human enamel.

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References

Fincham A, Moradian-Oldak J, Simmer J . The structural biology of the developing dental enamel matrix. J Struct Biol. 1999; 126(3):270-99. DOI: 10.1006/jsbi.1999.4130. View

Cochrane N, Cai F, Huq N, Burrow M, Reynolds E . New approaches to enhanced remineralization of tooth enamel. J Dent Res. 2010; 89(11):1187-97. DOI: 10.1177/0022034510376046. View

Kirkham J, Firth A, Vernals D, Boden N, Robinson C, Shore R . Self-assembling peptide scaffolds promote enamel remineralization. J Dent Res. 2007; 86(5):426-30. DOI: 10.1177/154405910708600507. View

Yang X, Sun Z, Ma R, Fan D, Moradian-Oldak J . Amelogenin "nanorods" formation during proteolysis by Mmp-20. J Struct Biol. 2011; 176(2):220-8. PMC: 3185149. DOI: 10.1016/j.jsb.2011.07.016. View

Fincham A, Moradian-Oldak J, Simmer J, Sarte P, Lau E, Diekwisch T . Self-assembly of a recombinant amelogenin protein generates supramolecular structures. J Struct Biol. 1994; 112(2):103-9. DOI: 10.1006/jsbi.1994.1011. View

Fan D, Du C, Sun Z, Lakshminarayanan R, Moradian-Oldak J . In vitro study on the interaction between the 32 kDa enamelin and amelogenin. J Struct Biol. 2009; 166(1):88-94. PMC: 4507495. DOI: 10.1016/j.jsb.2009.01.003. View

Shaw W, Campbell A, Paine M, Snead M . The COOH terminus of the amelogenin, LRAP, is oriented next to the hydroxyapatite surface. J Biol Chem. 2004; 279(39):40263-6. DOI: 10.1074/jbc.C400322200. View

Ravindranath R, Moradian-Oldak J, Fincham A . Tyrosyl motif in amelogenins binds N-acetyl-D-glucosamine. J Biol Chem. 1999; 274(4):2464-71. DOI: 10.1074/jbc.274.4.2464. View

Aoba T, Moreno E, Kresak M, Tanabe T . Possible roles of partial sequences at N- and C-termini of amelogenin in protein-enamel mineral interaction. J Dent Res. 1989; 68(9):1331-6. DOI: 10.1177/00220345890680090901. View

10.

Masuya H, Shimizu K, Sezutsu H, Sakuraba Y, Nagano J, Shimizu A . Enamelin (Enam) is essential for amelogenesis: ENU-induced mouse mutants as models for different clinical subtypes of human amelogenesis imperfecta (AI). Hum Mol Genet. 2005; 14(5):575-83. DOI: 10.1093/hmg/ddi054. View

11.

Cui F, Ge J . New observations of the hierarchical structure of human enamel, from nanoscale to microscale. J Tissue Eng Regen Med. 2007; 1(3):185-91. DOI: 10.1002/term.21. View

12.

Diekwisch T, David S, Bringas Jr P, Santos V, Slavkin H . Antisense inhibition of AMEL translation demonstrates supramolecular controls for enamel HAP crystal growth during embryonic mouse molar development. Development. 1993; 117(2):471-82. DOI: 10.1242/dev.117.2.471. View

13.

Iijima M, Du C, Abbott C, Doi Y, Moradian-Oldak J . Control of apatite crystal growth by the co-operative effect of a recombinant porcine amelogenin and fluoride. Eur J Oral Sci. 2006; 114 Suppl 1:304-7. DOI: 10.1111/j.1600-0722.2006.00324.x. View

14.

Ruan Q, Siddiqah N, Li X, Nutt S, Moradian-Oldak J . Amelogenin-chitosan matrix for human enamel regrowth: effects of viscosity and supersaturation degree. Connect Tissue Res. 2014; 55 Suppl 1:150-4. PMC: 4402213. DOI: 10.3109/03008207.2014.923856. View

15.

Wang L, Guan X, Du C, Moradian-Oldak J, Nancollas G . Amelogenin Promotes the Formation of Elongated Apatite Microstructures in a Controlled Crystallization System. J Phys Chem C Nanomater Interfaces. 2010; 111(17):6398-6404. PMC: 2843430. DOI: 10.1021/jp0675429. View

16.

Bajaj D, Arola D . On the R-curve behavior of human tooth enamel. Biomaterials. 2009; 30(23-24):4037-46. PMC: 2700976. DOI: 10.1016/j.biomaterials.2009.04.017. View

17.

Moradian-Oldak J . Amelogenins: assembly, processing and control of crystal morphology. Matrix Biol. 2001; 20(5-6):293-305. DOI: 10.1016/s0945-053x(01)00154-8. View

18.

Paine M, Snead M . Protein interactions during assembly of the enamel organic extracellular matrix. J Bone Miner Res. 1997; 12(2):221-7. DOI: 10.1359/jbmr.1997.12.2.221. View

19.

Ravindranath H, Chen L, Zeichner-David M, Ishima R, Ravindranath R . Interaction between the enamel matrix proteins amelogenin and ameloblastin. Biochem Biophys Res Commun. 2004; 323(3):1075-83. DOI: 10.1016/j.bbrc.2004.08.207. View

20.

Chen M, Yang J, Li J, Liang K, He L, Lin Z . Modulated regeneration of acid-etched human tooth enamel by a functionalized dendrimer that is an analog of amelogenin. Acta Biomater. 2014; 10(10):4437-46. DOI: 10.1016/j.actbio.2014.05.016. View