MicroED Data Collection and Processing

Overview

Journal Acta Crystallogr A Found Adv

Specialty Chemistry

Date 2015 Jul 2

PMID 26131894

Citations 77

Authors

Johan Hattne

Francis E Reyes

Brent L Nannenga

Dan Shi

M Jason de la Cruz

Andrew G W Leslie

Tamir Gonen

Affiliations

Soon will be listed here.

Abstract

MicroED, a method at the intersection of X-ray crystallography and electron cryo-microscopy, has rapidly progressed by exploiting advances in both fields and has already been successfully employed to determine the atomic structures of several proteins from sub-micron-sized, three-dimensional crystals. A major limiting factor in X-ray crystallography is the requirement for large and well ordered crystals. By permitting electron diffraction patterns to be collected from much smaller crystals, or even single well ordered domains of large crystals composed of several small mosaic blocks, MicroED has the potential to overcome the limiting size requirement and enable structural studies on difficult-to-crystallize samples. This communication details the steps for sample preparation, data collection and reduction necessary to obtain refined, high-resolution, three-dimensional models by MicroED, and presents some of its unique challenges.

Citing Articles

Fast event-based electron counting for small-molecule structure determination by MicroED.

Vlahakis N, Qu S, Richards L, Moraes L, Cascio D, Nelson H Acta Crystallogr C Struct Chem. 2025; 81(Pt 3):116-130.

PMID: 39982366 PMC: 11881165. DOI: 10.1107/S2053229624012300.

Accounting for electron-beam-induced warping of molecular nanocrystals in MicroED structure determination.

Vlahakis N, Clauss A, Rodriguez J IUCrJ. 2025; 12(Pt 2):223-238.

PMID: 39927752 PMC: 11878443. DOI: 10.1107/S2052252524012132.

Comprehensive microcrystal electron diffraction sample preparation for cryo-EM.

Nicolas W, Gillman C, Weaver S, Clabbers M, Shiriaeva A, Her A Nat Protoc. 2024; .

PMID: 39706914 DOI: 10.1038/s41596-024-01088-7.

An Updated Structure of Oxybutynin Hydrochloride.

Lin J, Bu G, Unge J, Gonen T Adv Sci (Weinh). 2024; 11(40):e2406494.

PMID: 39225443 PMC: 11515901. DOI: 10.1002/advs.202406494.

MicroED structure of the C11 cysteine protease clostripain.

Ruma Y, Bu G, Hattne J, Gonen T J Struct Biol X. 2024; 10:100107.

PMID: 39100863 PMC: 11296011. DOI: 10.1016/j.yjsbx.2024.100107.

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