Direct Wavefront Sensing for High-resolution in Vivo Imaging in Scattering Tissue

Overview

Journal Nat Commun

Specialty Biology

Date 2015 Jun 16

PMID 26073070

Citations 106

Authors

Kai Wang

Wenzhi Sun

Christopher T Richie

Brandon K Harvey

Eric Betzig

Na Ji

Affiliations

Soon will be listed here.

Abstract

Adaptive optics by direct imaging of the wavefront distortions of a laser-induced guide star has long been used in astronomy, and more recently in microscopy to compensate for aberrations in transparent specimens. Here we extend this approach to tissues that strongly scatter visible light by exploiting the reduced scattering of near-infrared guide stars. The method enables in vivo two-photon morphological and functional imaging down to 700 μm inside the mouse brain.

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