Experimental Characterization of an Isoplanatic Patch in Mouse Cortex Using Adaptive Optics

Overview

Journal Biomed Opt Express

Specialty Radiology

Date 2024 Oct 18

PMID 39421772

Authors

Jean Commere

Marie Glanc

Laurent Bourdieu

Raphael Galicher

Eric Gendron

Gerard Rousset

Affiliations

Soon will be listed here.

Abstract

Optical microscopy techniques have become essential tools for studying normal and pathological biological systems. However, in many situations, image quality deteriorates rapidly in the field of view due to optical aberrations and scattering induced by thick tissues. To compensate for these aberrations and restore the microscope's image quality, adaptive optics (AO) techniques have been proposed for the past 15 years. A key parameter for the AO implementation lies in the limited isoplanatic dimension over which the image quality remains uniform. Here, we propose a method for measuring this dimension and deducing the anisoplanatism and intensity transmission of the samples. We apply this approach to fixed slices of mouse cortices as a function of their thickness. We find a typical mid-maximum width of 20 µm for the isoplanatic spot, which is independent of sample thickness.

References

Lecoq J, Orlova N, Grewe B . Wide. Fast. Deep: Recent Advances in Multiphoton Microscopy of Neuronal Activity. J Neurosci. 2019; 39(46):9042-9052. PMC: 6855689. DOI: 10.1523/JNEUROSCI.1527-18.2019. View

Park J, Kong L, Zhou Y, Cui M . Large-field-of-view imaging by multi-pupil adaptive optics. Nat Methods. 2017; 14(6):581-583. PMC: 5482233. DOI: 10.1038/nmeth.4290. View

Sun W, Tan Z, Mensh B, Ji N . Thalamus provides layer 4 of primary visual cortex with orientation- and direction-tuned inputs. Nat Neurosci. 2015; 19(2):308-15. PMC: 4731241. DOI: 10.1038/nn.4196. View

Lacin M, Yildirim M . Applications of multiphoton microscopy in imaging cerebral and retinal organoids. Front Neurosci. 2024; 18:1360482. PMC: 10948410. DOI: 10.3389/fnins.2024.1360482. View

Ntziachristos V . Going deeper than microscopy: the optical imaging frontier in biology. Nat Methods. 2010; 7(8):603-14. DOI: 10.1038/nmeth.1483. View

Wang K, Milkie D, Saxena A, Engerer P, Misgeld T, Bronner M . Rapid adaptive optical recovery of optimal resolution over large volumes. Nat Methods. 2014; 11(6):625-8. PMC: 4069208. DOI: 10.1038/nmeth.2925. View

Hubert A, Harms F, Juvenal R, Treimany P, Levecq X, Loriette V . Adaptive optics light-sheet microscopy based on direct wavefront sensing without any guide star. Opt Lett. 2019; 44(10):2514-2517. DOI: 10.1364/OL.44.002514. View

Tao X, Azucena O, Fu M, Zuo Y, Chen D, Kubby J . Adaptive optics microscopy with direct wavefront sensing using fluorescent protein guide stars. Opt Lett. 2011; 36(17):3389-91. DOI: 10.1364/OL.36.003389. View

Yang W, Yuste R . In vivo imaging of neural activity. Nat Methods. 2017; 14(4):349-359. PMC: 5903578. DOI: 10.1038/nmeth.4230. View

10.

Wang K, Sun W, Richie C, Harvey B, Betzig E, Ji N . Direct wavefront sensing for high-resolution in vivo imaging in scattering tissue. Nat Commun. 2015; 6:7276. PMC: 4490402. DOI: 10.1038/ncomms8276. View

11.

Zeng J, Mahou P, Schanne-Klein M, Beaurepaire E, Debarre D . 3D resolved mapping of optical aberrations in thick tissues. Biomed Opt Express. 2012; 3(8):1898-913. PMC: 3409708. DOI: 10.1364/BOE.3.001898. View

12.

Rodriguez C, Ji N . Adaptive optical microscopy for neurobiology. Curr Opin Neurobiol. 2018; 50:83-91. PMC: 5984664. DOI: 10.1016/j.conb.2018.01.011. View

13.

Debarre D, Botcherby E, Watanabe T, Srinivas S, Booth M, Wilson T . Image-based adaptive optics for two-photon microscopy. Opt Lett. 2009; 34(16):2495-7. PMC: 3320043. DOI: 10.1364/ol.34.002495. View

14.

Wang C, Liu R, Milkie D, Sun W, Tan Z, Kerlin A . Multiplexed aberration measurement for deep tissue imaging in vivo. Nat Methods. 2014; 11(10):1037-40. PMC: 4180771. DOI: 10.1038/nmeth.3068. View

15.

Ji N . The practical and fundamental limits of optical imaging in mammalian brains. Neuron. 2014; 83(6):1242-5. DOI: 10.1016/j.neuron.2014.08.009. View

16.

Imperato S, Harms F, Hubert A, Mercier M, Bourdieu L, Fragola A . Single-shot quantitative aberration and scattering length measurements in mouse brain tissues using an extended-source Shack-Hartmann wavefront sensor. Opt Express. 2022; 30(9):15250-15265. DOI: 10.1364/OE.456651. View

17.

Rodriguez C, Chen A, Rivera J, Mohr M, Liang Y, Natan R . An adaptive optics module for deep tissue multiphoton imaging in vivo. Nat Methods. 2021; 18(10):1259-1264. PMC: 9090585. DOI: 10.1038/s41592-021-01279-0. View

18.

Liu R, Li Z, Marvin J, Kleinfeld D . Direct wavefront sensing enables functional imaging of infragranular axons and spines. Nat Methods. 2019; 16(7):615-618. PMC: 6763395. DOI: 10.1038/s41592-019-0434-7. View