Near-infrared Fluorescent Proteins for Multicolor in Vivo Imaging

Overview

Journal Nat Methods

Specialties Biomedical Engineering
Pathology

Date 2013 Jun 18

PMID 23770755

Citations 273

Authors

Daria M Shcherbakova

Vladislav V Verkhusha

Affiliations

Soon will be listed here.

Abstract

Near-infrared fluorescent proteins (FPs) are in high demand for in vivo imaging. We developed four spectrally distinct near-infrared FPs--iRFP670, iRFP682, iRFP702 and iRFP720--from bacterial phytochromes. iRFPs exhibit high brightness in mammalian cells and tissues and are suitable for long-term studies. iRFP670 and iRFP720 enable two-color imaging with standard approaches in living cells and mice. The four new iRFPs and the previously engineered iRFP713 allow multicolor imaging with spectral unmixing in living mice.

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References

Giraud E, Zappa S, Vuillet L, Adriano J, Hannibal L, Fardoux J . A new type of bacteriophytochrome acts in tandem with a classical bacteriophytochrome to control the antennae synthesis in Rhodopseudomonas palustris. J Biol Chem. 2005; 280(37):32389-97. DOI: 10.1074/jbc.M506890200. View

Auldridge M, Forest K . Bacterial phytochromes: more than meets the light. Crit Rev Biochem Mol Biol. 2011; 46(1):67-88. DOI: 10.3109/10409238.2010.546389. View

Shu X, Royant A, Lin M, Aguilera T, Lev-Ram V, Steinbach P . Mammalian expression of infrared fluorescent proteins engineered from a bacterial phytochrome. Science. 2009; 324(5928):804-7. PMC: 2763207. DOI: 10.1126/science.1168683. View

Giraud E, Vermeglio A . Bacteriophytochromes in anoxygenic photosynthetic bacteria. Photosynth Res. 2008; 97(2):141-53. DOI: 10.1007/s11120-008-9323-0. View

Weissleder R . A clearer vision for in vivo imaging. Nat Biotechnol. 2001; 19(4):316-7. DOI: 10.1038/86684. View

Ho S, Hunt H, Horton R, Pullen J, Pease L . Site-directed mutagenesis by overlap extension using the polymerase chain reaction. Gene. 1989; 77(1):51-9. DOI: 10.1016/0378-1119(89)90358-2. View

Subach O, Gundorov I, Yoshimura M, Subach F, Zhang J, Gruenwald D . Conversion of red fluorescent protein into a bright blue probe. Chem Biol. 2008; 15(10):1116-24. PMC: 2585067. DOI: 10.1016/j.chembiol.2008.08.006. View

Shcherbo D, Shemiakina I, Ryabova A, Luker K, Schmidt B, Souslova E . Near-infrared fluorescent proteins. Nat Methods. 2010; 7(10):827-9. PMC: 4425247. DOI: 10.1038/nmeth.1501. View

Auldridge M, Satyshur K, Anstrom D, Forest K . Structure-guided engineering enhances a phytochrome-based infrared fluorescent protein. J Biol Chem. 2012; 287(10):7000-9. PMC: 3293566. DOI: 10.1074/jbc.M111.295121. View

10.

Wagner J, Brunzelle J, Forest K, Vierstra R . A light-sensing knot revealed by the structure of the chromophore-binding domain of phytochrome. Nature. 2005; 438(7066):325-31. DOI: 10.1038/nature04118. View

11.

Piatkevich K, Subach F, Verkhusha V . Engineering of bacterial phytochromes for near-infrared imaging, sensing, and light-control in mammals. Chem Soc Rev. 2013; 42(8):3441-52. PMC: 3618476. DOI: 10.1039/c3cs35458j. View

12.

Strack R, Hein B, Bhattacharyya D, Hell S, Keenan R, Glick B . A rapidly maturing far-red derivative of DsRed-Express2 for whole-cell labeling. Biochemistry. 2009; 48(35):8279-81. PMC: 2861903. DOI: 10.1021/bi900870u. View

13.

Lin M, McKeown M, Ng H, Aguilera T, Shaner N, Campbell R . Autofluorescent proteins with excitation in the optical window for intravital imaging in mammals. Chem Biol. 2009; 16(11):1169-79. PMC: 2814181. DOI: 10.1016/j.chembiol.2009.10.009. View

14.

Filonov G, Krumholz A, Xia J, Yao J, Wang L, Verkhusha V . Deep-tissue photoacoustic tomography of a genetically encoded near-infrared fluorescent probe. Angew Chem Int Ed Engl. 2012; 51(6):1448-51. PMC: 3293502. DOI: 10.1002/anie.201107026. View

15.

Filonov G, Piatkevich K, Ting L, Zhang J, Kim K, Verkhusha V . Bright and stable near-infrared fluorescent protein for in vivo imaging. Nat Biotechnol. 2011; 29(8):757-61. PMC: 3152693. DOI: 10.1038/nbt.1918. View

16.

Wegerer A, Sun T, Altenbuchner J . Optimization of an E. coli L-rhamnose-inducible expression vector: test of various genetic module combinations. BMC Biotechnol. 2008; 8:2. PMC: 2254391. DOI: 10.1186/1472-6750-8-2. View

17.

Waddington S, McVey J, Bhella D, Parker A, Barker K, Atoda H . Adenovirus serotype 5 hexon mediates liver gene transfer. Cell. 2008; 132(3):397-409. DOI: 10.1016/j.cell.2008.01.016. View

18.

Giraud E, Zappa S, Jaubert M, Hannibal L, Fardoux J, Adriano J . Bacteriophytochrome and regulation of the synthesis of the photosynthetic apparatus in Rhodopseudomonas palustris: pitfalls of using laboratory strains. Photochem Photobiol Sci. 2004; 3(6):587-91. DOI: 10.1039/b315770a. View

19.

Kremers G, Goedhart J, van den Heuvel D, Gerritsen H, Gadella Jr T . Improved green and blue fluorescent proteins for expression in bacteria and mammalian cells. Biochemistry. 2007; 46(12):3775-83. DOI: 10.1021/bi0622874. View

20.

Subach O, Patterson G, Ting L, Wang Y, Condeelis J, Verkhusha V . A photoswitchable orange-to-far-red fluorescent protein, PSmOrange. Nat Methods. 2011; 8(9):771-7. PMC: 3164916. DOI: 10.1038/nmeth.1664. View