Colitogenic Bacteroides Thetaiotaomicron Antigens Access Host Immune Cells in a Sulfatase-Dependent Manner Via Outer Membrane Vesicles

Overview

Journal Cell Host Microbe

Publisher Cell Press

Specialties Infectious Diseases
Microbiology

Date 2015 May 15

PMID 25974305

Citations 130

Authors

Christina A Hickey

Kristine A Kuhn

David L Donermeyer

Nathan T Porter

Chunsheng Jin

Elizabeth A Cameron

Haerin Jung

Gerard E Kaiko

Marta Wegorzewska

Nicole P Malvin

Robert W P Glowacki

Gunnar C Hansson

Paul M Allen

Eric C Martens

Thaddeus S Stappenbeck

Affiliations

Soon will be listed here.

Abstract

Microbes interact with the host immune system via several potential mechanisms. One essential step for each mechanism is the method by which intestinal microbes or their antigens access specific host immune cells. Using genetically susceptible mice (dnKO) that develop spontaneous, fulminant colitis, triggered by Bacteroides thetaiotaomicron (B. theta), we investigated the mechanism of intestinal microbial access under conditions that stimulate colonic inflammation. B. theta antigens localized to host immune cells through outer membrane vesicles (OMVs) that harbor bacterial sulfatase activity. We deleted the anaerobic sulfatase maturating enzyme (anSME) from B. theta, which is required for post-translational activation of all B. theta sulfatase enzymes. This bacterial mutant strain did not stimulate colitis in dnKO mice. Lastly, access of B. theta OMVs to host immune cells was sulfatase dependent. These data demonstrate that bacterial OMVs and associated enzymes promote inflammatory immune stimulation in genetically susceptible hosts.

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