Biomimetic Extracellular Matrix Mediated Somatic Stem Cell Differentiation: Applications in Dental Pulp Tissue Regeneration

Overview

Journal Front Physiol

Date 2015 May 9

PMID 25954205

Citations 18

Authors

Sriram Ravindran

Anne George

Affiliations

Soon will be listed here.

Abstract

Dental caries is one of the most widely prevalent infectious diseases in the world. It affects more than half of the world's population. The current treatment for necrotic dental pulp tissue arising from dental caries is root canal therapy. This treatment results in loss of tooth sensitivity and vitality making it prone for secondary infections. Over the past decade, several tissue-engineering approaches have attempted regeneration of the dental pulp tissue. Although several studies have highlighted the potential of dental stem cells, none have transitioned into a clinical setting owing to limited availability of dental stem cells and the need for growth factor delivery systems. Our strategy is to utilize the intact ECM of pulp cells to drive lineage specific differentiation of bone marrow derived mesenchymal stem cells. From a clinical perspective, pulp ECM scaffolds can be generated using cell lines and patient specific somatic stem cells can be used for regeneration. Our published results have shown the feasibility of using pulp ECM scaffolds for odontogenic differentiation of non-dental mesenchymal cells. This focused review discusses the issues surrounding dental pulp tissue regeneration and the potential of our strategy to overcome these issues.

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References

Nakahara H, Misawa H, Hayashi T, Kondo E, Yuasa T, Kubota Y . Bone repair by transplantation of hTERT-immortalized human mesenchymal stem cells in mice. Transplantation. 2009; 88(3):346-53. DOI: 10.1097/TP.0b013e3181ae5ba2. View

Huang G, Gronthos S, Shi S . Mesenchymal stem cells derived from dental tissues vs. those from other sources: their biology and role in regenerative medicine. J Dent Res. 2009; 88(9):792-806. PMC: 2830488. DOI: 10.1177/0022034509340867. View

Yu J, Wang Y, Deng Z, Tang L, Li Y, Shi J . Odontogenic capability: bone marrow stromal stem cells versus dental pulp stem cells. Biol Cell. 2007; 99(8):465-74. DOI: 10.1042/BC20070013. View

Karaoz E, Cetinalp Demircan P, Saglam O, Aksoy A, Kaymaz F, Duruksu G . Human dental pulp stem cells demonstrate better neural and epithelial stem cell properties than bone marrow-derived mesenchymal stem cells. Histochem Cell Biol. 2011; 136(4):455-73. DOI: 10.1007/s00418-011-0858-3. View

Nakashima M, Reddi A . The application of bone morphogenetic proteins to dental tissue engineering. Nat Biotechnol. 2003; 21(9):1025-32. DOI: 10.1038/nbt864. View

Demarco F, Casagrande L, Zhang Z, Dong Z, Tarquinio S, Zeitlin B . Effects of morphogen and scaffold porogen on the differentiation of dental pulp stem cells. J Endod. 2010; 36(11):1805-11. DOI: 10.1016/j.joen.2010.08.031. View

Gronthos S, Brahim J, Li W, Fisher L, Cherman N, Boyde A . Stem cell properties of human dental pulp stem cells. J Dent Res. 2002; 81(8):531-5. DOI: 10.1177/154405910208100806. View

Ravindran S, Gao Q, Kotecha M, Magin R, Karol S, Bedran-Russo A . Biomimetic extracellular matrix-incorporated scaffold induces osteogenic gene expression in human marrow stromal cells. Tissue Eng Part A. 2011; 18(3-4):295-309. PMC: 3267968. DOI: 10.1089/ten.TEA.2011.0136. View

Levorson E, Hu O, Mountziaris P, Kasper F, Mikos A . Cell-derived polymer/extracellular matrix composite scaffolds for cartilage regeneration, Part 2: construct devitalization and determination of chondroinductive capacity. Tissue Eng Part C Methods. 2013; 20(4):358-72. PMC: 3968877. DOI: 10.1089/ten.tec.2013.0288. View

10.

Feng R, Lengner C . Application of Stem Cell Technology in Dental Regenerative Medicine. Adv Wound Care (New Rochelle). 2014; 2(6):296-305. PMC: 3842883. DOI: 10.1089/wound.2012.0375. View

11.

Shi S, Robey P, Gronthos S . Comparison of human dental pulp and bone marrow stromal stem cells by cDNA microarray analysis. Bone. 2001; 29(6):532-9. DOI: 10.1016/s8756-3282(01)00612-3. View

12.

DAquino R, De Rosa A, Lanza V, Tirino V, Laino L, Graziano A . Human mandible bone defect repair by the grafting of dental pulp stem/progenitor cells and collagen sponge biocomplexes. Eur Cell Mater. 2009; 18:75-83. DOI: 10.22203/ecm.v018a07. View

13.

Langer R, Vacanti J . Tissue engineering. Science. 1993; 260(5110):920-6. DOI: 10.1126/science.8493529. View

14.

Vo T, Kasper F, Mikos A . Strategies for controlled delivery of growth factors and cells for bone regeneration. Adv Drug Deliv Rev. 2012; 64(12):1292-309. PMC: 3358582. DOI: 10.1016/j.addr.2012.01.016. View

15.

Ehninger A, Trumpp A . The bone marrow stem cell niche grows up: mesenchymal stem cells and macrophages move in. J Exp Med. 2011; 208(3):421-8. PMC: 3058583. DOI: 10.1084/jem.20110132. View

16.

Ramachandran A, Ravindran S, George A . Localization of transforming growth factor beta receptor II interacting protein-1 in bone and teeth: implications in matrix mineralization. J Histochem Cytochem. 2012; 60(4):323-37. PMC: 3351241. DOI: 10.1369/0022155412436879. View

17.

Bourget J, Gauvin R, Larouche D, Lavoie A, Labbe R, Auger F . Human fibroblast-derived ECM as a scaffold for vascular tissue engineering. Biomaterials. 2012; 33(36):9205-13. DOI: 10.1016/j.biomaterials.2012.09.015. View

18.

Ravindran S, George A . Multifunctional ECM proteins in bone and teeth. Exp Cell Res. 2014; 325(2):148-54. PMC: 4072740. DOI: 10.1016/j.yexcr.2014.01.018. View

19.

Alongi D, Yamaza T, Song Y, Fouad A, Romberg E, Shi S . Stem/progenitor cells from inflamed human dental pulp retain tissue regeneration potential. Regen Med. 2010; 5(4):617-31. PMC: 3035701. DOI: 10.2217/rme.10.30. View

20.

Rosa V, Zhang Z, Grande R, Nor J . Dental pulp tissue engineering in full-length human root canals. J Dent Res. 2013; 92(11):970-5. PMC: 3797540. DOI: 10.1177/0022034513505772. View