Localization of Transforming Growth Factor Beta Receptor II Interacting Protein-1 in Bone and Teeth: Implications in Matrix Mineralization

Overview

Journal J Histochem Cytochem

Publisher Sage Publications

Specialty Biochemistry

Date 2012 Jan 21

PMID 22260994

Citations 8

Authors

Amsaveni Ramachandran

Sriram Ravindran

Anne George

Affiliations

Soon will be listed here.

Abstract

Transforming growth factor beta receptor II (TGFβR-II) interacting protein 1 (TRIP-1) is a WD-40 protein that binds to the cytoplasmic domain of the TGF-β type II receptor in a kinase-dependent manner. To investigate the role of TRIP-1 in mineralized tissues, we examined its pattern of expression in cartilage, bone, and teeth and analyzed the relationship between TRIP-1 overexpression and mineralized matrix formation. Results demonstrate that TRIP-1 was predominantly expressed by osteoblasts, odontoblasts, and chondrocytes in these tissues. Interestingly, TRIP-1 was also localized in the extracellular matrix of bone and at the mineralization front in dentin, suggesting that TRIP-1 is secreted by nonclassical secretory mechanisms, as it is devoid of a signal peptide. In vitro nucleation studies demonstrate a role for TRIP-1 in nucleating calcium phosphate polymorphs. Overexpression of TRIP-1 favored osteoblast differentiation of undifferentiated mesenchymal cells with an increase in mineralized matrix formation. These data indicate an unexpected role for TRIP-1 during development of bone, teeth, and cartilage.

Citing Articles

Cellular Signaling for Dental Physiological Functions.

Ouchi T, Nakagawa T Biomolecules. 2023; 13(8).

PMID: 37627242 PMC: 10452277. DOI: 10.3390/biom13081177.

Biomineralization of Enamel and Dentin Mediated by Matrix Proteins.

Moradian-Oldak J, George A J Dent Res. 2021; 100(10):1020-1029.

PMID: 34151644 PMC: 8381691. DOI: 10.1177/00220345211018405.

STIM1 a calcium sensor promotes the assembly of an ECM that contains Extracellular vesicles and factors that modulate mineralization.

Chen Y, Koshy R, Guirado E, George A Acta Biomater. 2020; 120:224-239.

PMID: 33130308 PMC: 7796999. DOI: 10.1016/j.actbio.2020.10.011.

TRIP-1 Promotes the Assembly of an ECM That Contains Extracellular Vesicles and Factors That Modulate Angiogenesis.

Chen Y, George A Front Physiol. 2018; 9:1092.

PMID: 30158875 PMC: 6104305. DOI: 10.3389/fphys.2018.01092.

TGF beta receptor II interacting protein-1, an intracellular protein has an extracellular role as a modulator of matrix mineralization.

Ramachandran A, Ravindran S, Huang C, George A Sci Rep. 2016; 6:37885.

PMID: 27883077 PMC: 5121659. DOI: 10.1038/srep37885.

References

Sasaki T, Ito Y, Bringas Jr P, Chou S, Urata M, Slavkin H . TGFbeta-mediated FGF signaling is crucial for regulating cranial neural crest cell proliferation during frontal bone development. Development. 2005; 133(2):371-81. DOI: 10.1242/dev.02200. View

Chen R, Miettinen P, Maruoka E, Choy L, Derynck R . A WD-domain protein that is associated with and phosphorylated by the type II TGF-beta receptor. Nature. 1995; 377(6549):548-52. DOI: 10.1038/377548a0. View

Asano K, Kinzy T, Merrick W, Hershey J . Conservation and diversity of eukaryotic translation initiation factor eIF3. J Biol Chem. 1997; 272(2):1101-9. DOI: 10.1074/jbc.272.2.1101. View

Ravindran S, Gao Q, Kotecha M, Magin R, Karol S, Bedran-Russo A . Biomimetic extracellular matrix-incorporated scaffold induces osteogenic gene expression in human marrow stromal cells. Tissue Eng Part A. 2011; 18(3-4):295-309. PMC: 3267968. DOI: 10.1089/ten.TEA.2011.0136. View

Smith T, Gaitatzes C, Saxena K, Neer E . The WD repeat: a common architecture for diverse functions. Trends Biochem Sci. 1999; 24(5):181-5. DOI: 10.1016/s0968-0004(99)01384-5. View

Perez R, Navarro A, Rezaiekhaligh M, Mabry S, Ekekezie I . TRIP-1 regulates TGF-β1-induced epithelial-mesenchymal transition of human lung epithelial cell line A549. Am J Physiol Lung Cell Mol Physiol. 2011; 300(5):L799-807. DOI: 10.1152/ajplung.00350.2010. View

Ahlemann M, Zeidler R, Lang S, Mack B, Munz M, Gires O . Carcinoma-associated eIF3i overexpression facilitates mTOR-dependent growth transformation. Mol Carcinog. 2006; 45(12):957-67. DOI: 10.1002/mc.20269. View

Neer E, Schmidt C, Nambudripad R, Smith T . The ancient regulatory-protein family of WD-repeat proteins. Nature. 1994; 371(6495):297-300. DOI: 10.1038/371297a0. View

Hao J, Narayanan K, Ramachandran A, He G, Almushayt A, Evans C . Odontoblast cells immortalized by telomerase produce mineralized dentin-like tissue both in vitro and in vivo. J Biol Chem. 2002; 277(22):19976-81. DOI: 10.1074/jbc.M112223200. View

10.

Roberts A, Sporn M . Physiological actions and clinical applications of transforming growth factor-beta (TGF-beta). Growth Factors. 1993; 8(1):1-9. DOI: 10.3109/08977199309029129. View

11.

Kolupaeva V, Unbehaun A, Lomakin I, Hellen C, Pestova T . Binding of eukaryotic initiation factor 3 to ribosomal 40S subunits and its role in ribosomal dissociation and anti-association. RNA. 2005; 11(4):470-86. PMC: 1370736. DOI: 10.1261/rna.7215305. View

12.

Behlke J, Bommer U, LUTSCH G, HENSKE A, BIELKA H . Structure of initiation factor eIF-3 from rat liver. Hydrodynamic and electron microscopic investigations. Eur J Biochem. 1986; 157(3):523-30. DOI: 10.1111/j.1432-1033.1986.tb09698.x. View

13.

Gilissen C, Arts H, Hoischen A, Spruijt L, Mans D, Arts P . Exome sequencing identifies WDR35 variants involved in Sensenbrenner syndrome. Am J Hum Genet. 2010; 87(3):418-23. PMC: 2933349. DOI: 10.1016/j.ajhg.2010.08.004. View

14.

Massague J, Blain S, Lo R . TGFbeta signaling in growth control, cancer, and heritable disorders. Cell. 2000; 103(2):295-309. DOI: 10.1016/s0092-8674(00)00121-5. View

15.

Chai Y, Zhao J, Mogharei A, Xu B, Bringas Jr P, Shuler C . Inhibition of transforming growth factor-beta type II receptor signaling accelerates tooth formation in mouse first branchial arch explants. Mech Dev. 1999; 86(1-2):63-74. DOI: 10.1016/s0925-4773(99)00112-4. View

16.

Choy L, Derynck R . The type II transforming growth factor (TGF)-beta receptor-interacting protein TRIP-1 acts as a modulator of the TGF-beta response. J Biol Chem. 1998; 273(47):31455-62. DOI: 10.1074/jbc.273.47.31455. View

17.

Sheu T, Schwarz E, Martinez D, OKeefe R, Rosier R, Zuscik M . A phage display technique identifies a novel regulator of cell differentiation. J Biol Chem. 2002; 278(1):438-43. DOI: 10.1074/jbc.M208292200. View

18.

He G, George A . Dentin matrix protein 1 immobilized on type I collagen fibrils facilitates apatite deposition in vitro. J Biol Chem. 2003; 279(12):11649-56. DOI: 10.1074/jbc.M309296200. View

19.

Chiellini C, Cochet O, Negroni L, Samson M, Poggi M, Ailhaud G . Characterization of human mesenchymal stem cell secretome at early steps of adipocyte and osteoblast differentiation. BMC Mol Biol. 2008; 9:26. PMC: 2279142. DOI: 10.1186/1471-2199-9-26. View

20.

Ito Y, Yeo J, Chytil A, Han J, Bringas Jr P, Nakajima A . Conditional inactivation of Tgfbr2 in cranial neural crest causes cleft palate and calvaria defects. Development. 2003; 130(21):5269-80. DOI: 10.1242/dev.00708. View