Stem/progenitor Cells from Inflamed Human Dental Pulp Retain Tissue Regeneration Potential

Overview

Journal Regen Med

Specialty Biotechnology

Date 2010 May 15

PMID 20465527

Citations 104

Authors

Dominick J Alongi

Takayoshi Yamaza

Yingjie Song

Ashraf F Fouad

Elaine E Romberg

Songtao Shi

Rocky S Tuan

George T-J Huang

Affiliations

Soon will be listed here.

Abstract

Background: Potent stem/progenitor cells have been isolated from normal human dental pulps termed dental pulp stem cells (DPSCs). However, it is unknown whether these cells exist in inflamed pulps (IPs).

Aims: To determine whether DPSCs can be identified and isolated from IPs; and if they can be successfully cultured, whether they retain tissue regeneration potential in vivo.

Materials & Methods: DPSCs from freshly collected normal pulps (NPs) and IPs were characterized in vitro and their tissue regeneration potential tested using an in vivo study model.

Results: The immunohistochemical analysis showed that IPs expressed higher levels of mesenchymal stem cell markers STRO-1, CD90, CD105 and CD146 compared with NPs (p < 0.05). Flow cytometry analysis showed that DPSCs from both NPs and IPs expressed moderate to high levels of CD146, stage-specific embryonic antigen-4, CD73 and CD166. Total population doubling of DPSCs-IPs (44.6 + or - 2.9) was lower than that of DPSCs-NPs (58.9 + or - 2.5) (p < 0.05), and DPSCs-IPs appeared to have a decreased osteo/dentinogenic potential compared with DPSCs-NPs based on the mineral deposition in cultures. Nonetheless, DPSCs-IPs formed pulp/dentin complexes similar to DPSCs-NPs when transplanted into immunocompromised mice.

Conclusion: DPSCs-IPs can be isolated and their mesenchymal stem cell marker profiles are similar to those from NPs. Although some stem cell properties of DPSCs-IPs were altered, cells from some samples remained potent in tissue regeneration in vivo.

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References

Gronthos S, Brahim J, Li W, Fisher L, Cherman N, Boyde A . Stem cell properties of human dental pulp stem cells. J Dent Res. 2002; 81(8):531-5. DOI: 10.1177/154405910208100806. View

Sonoyama W, Liu Y, Yamaza T, Tuan R, Wang S, Shi S . Characterization of the apical papilla and its residing stem cells from human immature permanent teeth: a pilot study. J Endod. 2008; 34(2):166-71. PMC: 2714367. DOI: 10.1016/j.joen.2007.11.021. View

Huang A, Chen Y, Lin L, Shieh T, Chan A . Isolation and characterization of dental pulp stem cells from a supernumerary tooth. J Oral Pathol Med. 2008; 37(9):571-4. DOI: 10.1111/j.1600-0714.2008.00654.x. View

Pezelj-Ribaric S, Magasic K, Prpic J, Miletic I, Karlovic Z . Tumor necrosis factor-alpha in peripical tissue exudates of teeth with apical periodontitis. Mediators Inflamm. 2008; 2007:69416. PMC: 2248244. DOI: 10.1155/2007/69416. View

Paula-Silva F, Ghosh A, Silva L, Kapila Y . TNF-alpha promotes an odontoblastic phenotype in dental pulp cells. J Dent Res. 2009; 88(4):339-44. PMC: 3144054. DOI: 10.1177/0022034509334070. View

Garcia-Castro J, Trigueros C, Madrenas J, Perez-Simon J, Rodriguez R, Menendez P . Mesenchymal stem cells and their use as cell replacement therapy and disease modelling tool. J Cell Mol Med. 2009; 12(6B):2552-65. PMC: 3828873. DOI: 10.1111/j.1582-4934.2008.00516.x. View

Silva A, Faria M, Fontes A, Campos M, Cavalcanti B . Interleukin-1 beta and interleukin-8 in healthy and inflamed dental pulps. J Appl Oral Sci. 2009; 17(5):527-32. PMC: 4327685. DOI: 10.1590/s1678-77572009000500031. View

Song L, Webb N, Song Y, Tuan R . Identification and functional analysis of candidate genes regulating mesenchymal stem cell self-renewal and multipotency. Stem Cells. 2006; 24(7):1707-18. DOI: 10.1634/stemcells.2005-0604. View

Nakashima M, Iohara K, Ishikawa M, Ito M, Tomokiyo A, Tanaka T . Stimulation of reparative dentin formation by ex vivo gene therapy using dental pulp stem cells electrotransfected with growth/differentiation factor 11 (Gdf11). Hum Gene Ther. 2004; 15(11):1045-53. DOI: 10.1089/hum.2004.15.1045. View

10.

Li X, Makarov S . An essential role of NF-kappaB in the "tumor-like" phenotype of arthritic synoviocytes. Proc Natl Acad Sci U S A. 2006; 103(46):17432-7. PMC: 1859946. DOI: 10.1073/pnas.0607939103. View

11.

Sonoyama W, Liu Y, Fang D, Yamaza T, Seo B, Zhang C . Mesenchymal stem cell-mediated functional tooth regeneration in swine. PLoS One. 2006; 1:e79. PMC: 1762318. DOI: 10.1371/journal.pone.0000079. View

12.

Huang G . Pulp and dentin tissue engineering and regeneration: current progress. Regen Med. 2009; 4(5):697-707. PMC: 2785543. DOI: 10.2217/rme.09.45. View

13.

Huang G, Shagramanova K, Chan S . Formation of odontoblast-like cells from cultured human dental pulp cells on dentin in vitro. J Endod. 2006; 32(11):1066-73. DOI: 10.1016/j.joen.2006.05.009. View

14.

Nakashima M, Akamine A . The application of tissue engineering to regeneration of pulp and dentin in endodontics. J Endod. 2005; 31(10):711-8. DOI: 10.1097/01.don.0000164138.49923.e5. View

15.

Abbas S, Zhang Y, Clohisy J, Abu-Amer Y . Tumor necrosis factor-alpha inhibits pre-osteoblast differentiation through its type-1 receptor. Cytokine. 2003; 22(1-2):33-41. DOI: 10.1016/s1043-4666(03)00106-6. View

16.

Blazer S, Khankin E, Segev Y, Ofir R, Kra-Oz Z, Gottfried Y . High glucose-induced replicative senescence: point of no return and effect of telomerase. Biochem Biophys Res Commun. 2002; 296(1):93-101. DOI: 10.1016/s0006-291x(02)00818-5. View

17.

Mobasheri A, Csaki C, Clutterbuck A, Rahmanzadeh M, Shakibaei M . Mesenchymal stem cells in connective tissue engineering and regenerative medicine: applications in cartilage repair and osteoarthritis therapy. Histol Histopathol. 2009; 24(3):347-66. DOI: 10.14670/HH-24.347. View

18.

Reffelmann T, Konemann S, Kloner R . Promise of blood- and bone marrow-derived stem cell transplantation for functional cardiac repair: putting it in perspective with existing therapy. J Am Coll Cardiol. 2009; 53(4):305-8. DOI: 10.1016/j.jacc.2008.10.018. View

19.

Shi S, Gronthos S . Perivascular niche of postnatal mesenchymal stem cells in human bone marrow and dental pulp. J Bone Miner Res. 2003; 18(4):696-704. DOI: 10.1359/jbmr.2003.18.4.696. View

20.

Yamaza T, Miura Y, Akiyama K, Bi Y, Sonoyama W, Gronthos S . Mesenchymal stem cell-mediated ectopic hematopoiesis alleviates aging-related phenotype in immunocompromised mice. Blood. 2008; 113(11):2595-604. PMC: 2656279. DOI: 10.1182/blood-2008-10-182246. View