Chromosome Mis-segregation and Cytokinesis Failure in Trisomic Human Cells

Overview

Journal Elife

Specialty Biology

Date 2015 May 6

PMID 25942454

Citations 54

Authors

Joshua M Nicholson

Joana C Macedo

Aaron J Mattingly

Darawalee Wangsa

Jordi Camps

Vera Lima

Ana M Gomes

Sofia Doria

Thomas Ried

Elsa Logarinho

Daniela Cimini

Affiliations

Soon will be listed here.

Abstract

Cancer cells display aneuploid karyotypes and typically mis-segregate chromosomes at high rates, a phenotype referred to as chromosomal instability (CIN). To test the effects of aneuploidy on chromosome segregation and other mitotic phenotypes we used the colorectal cancer cell line DLD1 (2n = 46) and two variants with trisomy 7 or 13 (DLD1+7 and DLD1+13), as well as euploid and trisomy 13 amniocytes (AF and AF+13). We found that trisomic cells displayed higher rates of chromosome mis-segregation compared to their euploid counterparts. Furthermore, cells with trisomy 13 displayed a distinctive cytokinesis failure phenotype. We showed that up-regulation of SPG20 expression, brought about by trisomy 13 in DLD1+13 and AF+13 cells, is sufficient for the cytokinesis failure phenotype. Overall, our study shows that aneuploidy can induce chromosome mis-segregation. Moreover, we identified a trisomy 13-specific mitotic phenotype that is driven by up-regulation of a gene encoded on the aneuploid chromosome.

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