Emergence of KPC-2-producing Pseudomonas Aeruginosa Sequence Type 463 Isolates in Hangzhou, China

Overview

Journal Antimicrob Agents Chemother

Publisher American Society for Microbiology

Specialty Pharmacology

Date 2015 Feb 19

PMID 25691651

Citations 18

Authors

Yan-Yan Hu

Dan-Xia Gu

Jia-Chang Cai

Hong-Wei Zhou

Rong Zhang

Affiliations

Soon will be listed here.

Abstract

Thirty-nine Klebsiella pneumoniae carbapenemase (KPC)-producing Pseudomonas aeruginosa isolates, all exhibiting high-level resistance to carbapenems and other β-lactam antibiotics, were isolated in Hangzhou, China. Molecular epidemiology analysis indicated the presence of two dominant clones, namely, clones A and B, both of which belong to sequence type 463 (ST463). A genetic environment analysis demonstrated that both clones harbor an ISKpn8 transposase, bla(KPC-2), and an ISKpn6-like transposase. These findings depict the features of clonal expansion and transmission of KPC-2-producing P. aeruginosa strains in Hangzhou, China.

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References

Davies T, Queenan A, Morrow B, Shang W, Amsler K, He W . Longitudinal survey of carbapenem resistance and resistance mechanisms in Enterobacteriaceae and non-fermenters from the USA in 2007-09. J Antimicrob Chemother. 2011; 66(10):2298-307. DOI: 10.1093/jac/dkr290. View

Hu Y, Cai J, Zhang R, Zhou H, Sun Q, Chen G . Emergence of Proteus mirabilis harboring blaKPC-2 and qnrD in a Chinese Hospital. Antimicrob Agents Chemother. 2012; 56(5):2278-82. PMC: 3346625. DOI: 10.1128/AAC.05519-11. View

Edelstein M, Skleenova E, Shevchenko O, DSouza J, Tapalski D, Azizov I . Spread of extensively resistant VIM-2-positive ST235 Pseudomonas aeruginosa in Belarus, Kazakhstan, and Russia: a longitudinal epidemiological and clinical study. Lancet Infect Dis. 2013; 13(10):867-76. DOI: 10.1016/S1473-3099(13)70168-3. View

Cai J, Zhang R, Hu Y, Zhou H, Chen G . Emergence of Escherichia coli sequence type 131 isolates producing KPC-2 carbapenemase in China. Antimicrob Agents Chemother. 2013; 58(2):1146-52. PMC: 3910876. DOI: 10.1128/AAC.00912-13. View

Lister P, Wolter D, Hanson N . Antibacterial-resistant Pseudomonas aeruginosa: clinical impact and complex regulation of chromosomally encoded resistance mechanisms. Clin Microbiol Rev. 2009; 22(4):582-610. PMC: 2772362. DOI: 10.1128/CMR.00040-09. View

Zhang R, Hu Y, Yang X, Gu D, Zhou H, Hu Q . Emergence of NDM-producing non-baumannii Acinetobacter spp. isolated from China. Eur J Clin Microbiol Infect Dis. 2013; 33(5):853-60. DOI: 10.1007/s10096-013-2024-4. View

Yan J, Hsueh P, Ko W, Luh K, Tsai S, Wu H . Metallo-beta-lactamases in clinical Pseudomonas isolates in Taiwan and identification of VIM-3, a novel variant of the VIM-2 enzyme. Antimicrob Agents Chemother. 2001; 45(8):2224-8. PMC: 90635. DOI: 10.1128/AAC.45.8.2224-2228.2001. View

Carrico J, Pinto F, Simas C, Nunes S, Sousa N, Frazao N . Assessment of band-based similarity coefficients for automatic type and subtype classification of microbial isolates analyzed by pulsed-field gel electrophoresis. J Clin Microbiol. 2005; 43(11):5483-90. PMC: 1287802. DOI: 10.1128/JCM.43.11.5483-5490.2005. View

Cai J, Zhou H, Zhang R, Chen G . Emergence of Serratia marcescens, Klebsiella pneumoniae, and Escherichia coli Isolates possessing the plasmid-mediated carbapenem-hydrolyzing beta-lactamase KPC-2 in intensive care units of a Chinese hospital. Antimicrob Agents Chemother. 2008; 52(6):2014-8. PMC: 2415814. DOI: 10.1128/AAC.01539-07. View

10.

Shen P, Wei Z, Jiang Y, Du X, Ji S, Yu Y . Novel genetic environment of the carbapenem-hydrolyzing beta-lactamase KPC-2 among Enterobacteriaceae in China. Antimicrob Agents Chemother. 2009; 53(10):4333-8. PMC: 2764158. DOI: 10.1128/AAC.00260-09. View

11.

Yigit H, Queenan A, Anderson G, Domenech-Sanchez A, Biddle J, Steward C . Novel carbapenem-hydrolyzing beta-lactamase, KPC-1, from a carbapenem-resistant strain of Klebsiella pneumoniae. Antimicrob Agents Chemother. 2001; 45(4):1151-61. PMC: 90438. DOI: 10.1128/AAC.45.4.1151-1161.2001. View