Transposable Elements Can Be Used to Study Cell Lineages in Transgenic Plants

Overview

Journal Plant Cell

Publisher Oxford University Press

Specialties Biology
Cell Biology

Date 1989 Aug 1

PMID 2562511

Citations 20

Authors

E J Finnegan

B H Taylor

S Craig

E S Dennis

Affiliations

Soon will be listed here.

Abstract

The beta-glucuronidase reporter gene has been used to develop a sensitive assay for the excision of transposable elements introduced into transgenic plants. The reporter gene, inactivated by the insertion of the maize transposable element Activator (Ac) into the 5'-untranslated leader, was introduced into the genome of tobacco by Agrobacterium-mediated transformation. Reactivation of the beta-glucuronidase gene was detected in transgenic plants using a fluorometric or histochemical assay. Reactivation of the reporter gene was dependent on the presence of the transposase of Ac, and resulted from the excision of the Ac element. This assay, together with the improved methods for visualization, will provide a valuable and rapid method for studying the basic mechanism of transposition in plants and for developing modified transposable element systems suitable for gene tagging in transgenic plants.

Citing Articles

In situ detection of transposition of the maize controlling element (Ac) in transgenic soybean tissues.

Zhou J, Atherly A Plant Cell Rep. 2013; 8(9):542-5.

PMID: 24226282 DOI: 10.1007/BF00820205.

Developing a transposon tagging system to isolate rust-resistance genes from flax.

Ellis J, Finnegan E, Lawrence G Theor Appl Genet. 2013; 85(1):46-54.

PMID: 24197227 DOI: 10.1007/BF00223843.

Agrobacterium-mediated in vitro transformation of wood-producing stem segments in eucalypts.

Spokevicius A, Van Beveren K, Leitch M, Bossinger G Plant Cell Rep. 2004; 23(9):617-24.

PMID: 15322811 DOI: 10.1007/s00299-004-0856-1.

Transformation of Maize Cells and Regeneration of Fertile Transgenic Plants.

Gordon-Kamm W, Spencer T, Mangano M, Adams T, Daines R, Start W Plant Cell. 1990; 2(7):603-618.

PMID: 12354967 PMC: 159915. DOI: 10.1105/tpc.2.7.603.

Early and multiple Ac transpositions in rice suitable for efficient insertional mutagenesis.

Greco R, Ouwerkerk P, Taal A, Favalli C, Beguiristain T, Puigdomenech P Plant Mol Biol. 2001; 46(2):215-27.

PMID: 11442061 DOI: 10.1023/a:1010607318694.

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