The 17-kb Tam1 Element of Antirrhinum Majus Induces a 3-bp Duplication Upon Integration into the Chalcone Synthase Gene

Overview

Journal EMBO J

Specialties Biotechnology
Molecular Biology

Date 1984 May 1

PMID 16453519

Citations 45

Authors

U Bonas

H Sommer

H Saedler

Affiliations

Soon will be listed here.

Abstract

The DNA sequence of the termini and the flanking regions of the 17-kb transposable element Tam1 was determined. Tam1 is integrated in the chalcone synthase gene of the niv-53 mutant of Antirrhinum majus. The element has a 13-bp perfect inverted repeat at its termini and appears to induce a 3-bp duplication of the target site upon integration. The DNA sequence of a niv revertant was analyzed and found to differ from the wild-type sequence by an additional 2 bp that seem to derive from the target site duplication. Stretches of homologous sequences have been found between the ends of Tam1, within each terminus of the element, and between the termini and target site sequences. Structural similarities between the ends of Tam1 and the Spm-18 element of Zea mays reflect a possible horizontal spread of a common progenitor.

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Molecular analysis of paramutant plants of Antirrhinum majus and the involvement of transposable elements.

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