Binary Agrobacterium Vectors for Plant Transformation

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 1984 Nov 26

PMID 6095209

Citations 851

Authors

M Bevan

Affiliations

Soon will be listed here.

Abstract

A vector molecule for the efficient transformation of higher plants has been constructed with several features that make it efficient to use. It utilizes the trans acting functions of the vir region of a co-resident Ti plasmid in Agrobacterium tumefaciens to transfer sequences bordered by left and right T-DNA border sequences into the nuclear genome of plants. The T-region contains a dominant selectable marker gene that confers high levels of resistance to kanamycin, and a lac alpha-complementing region from M13mp19 that contains several unique restriction sites for the positive selection of inserted DNA.

Citing Articles

Engineering Agrobacterium for improved plant transformation.

Goralogia G, Willig C, Strauss S Plant J. 2025; 121(5):e70015.

PMID: 40051182 PMC: 11885899. DOI: 10.1111/tpj.70015.

COL2-dependent photoperiodic floral induction in seems to be lost in the × hybrid .

Schmidt F, Grundmann L, Lahme M, Seidemann M, Schwarze A, Lichtenauer S Front Plant Sci. 2024; 14:1249879.

PMID: 38239221 PMC: 10794312. DOI: 10.3389/fpls.2023.1249879.

Mitochondrial atp1 mRNA knockdown by a custom-designed pentatricopeptide repeat protein alters ATP synthase.

Yang F, Vincis Pereira Sanglard L, Lee C, Stroher E, Singh S, Oh G Plant Physiol. 2024; 194(4):2631-2647.

PMID: 38206203 PMC: 10980415. DOI: 10.1093/plphys/kiae008.

Development of constitutive and IPTG-inducible integron promoter-based expression systems for and .

Toh W, Teo Y, Tor X, Loh P, Wong H 3 Biotech. 2023; 13(3):91.

PMID: 36825259 PMC: 9941393. DOI: 10.1007/s13205-023-03507-0.

Development of a set of novel binary expression vectors for plant gene function analysis and genetic transformation.

Wang X, Teng C, Wei H, Liu S, Xuan H, Peng W Front Plant Sci. 2023; 13:1104905.

PMID: 36714700 PMC: 9877630. DOI: 10.3389/fpls.2022.1104905.

References

Ditta G, Stanfield S, Corbin D, Helinski D . Broad host range DNA cloning system for gram-negative bacteria: construction of a gene bank of Rhizobium meliloti. Proc Natl Acad Sci U S A. 1980; 77(12):7347-51. PMC: 350500. DOI: 10.1073/pnas.77.12.7347. View

Fraley R, Rogers S, Horsch R, Sanders P, Flick J, Adams S . Expression of bacterial genes in plant cells. Proc Natl Acad Sci U S A. 1983; 80(15):4803-7. PMC: 384133. DOI: 10.1073/pnas.80.15.4803. View

Herrera-Estrella L, Block M, Messens E, Hernalsteens J, Montagu M, Schell J . Chimeric genes as dominant selectable markers in plant cells. EMBO J. 1983; 2(6):987-95. PMC: 555219. DOI: 10.1002/j.1460-2075.1983.tb01532.x. View

Barton K, Binns A, Matzke A, Chilton M . Regeneration of intact tobacco plants containing full length copies of genetically engineered T-DNA, and transmission of T-DNA to R1 progeny. Cell. 1983; 32(4):1033-43. DOI: 10.1016/0092-8674(83)90288-x. View

Klee H, Gordon M, Nester E . Complementation analysis of Agrobacterium tumefaciens Ti plasmid mutations affecting oncogenicity. J Bacteriol. 1982; 150(1):327-31. PMC: 220117. DOI: 10.1128/jb.150.1.327-331.1982. View

Trieu-Cuot P, Courvalin P . Nucleotide sequence of the Streptococcus faecalis plasmid gene encoding the 3'5"-aminoglycoside phosphotransferase type III. Gene. 1983; 23(3):331-41. DOI: 10.1016/0378-1119(83)90022-7. View

Bevan M, Barnes W, Chilton M . Structure and transcription of the nopaline synthase gene region of T-DNA. Nucleic Acids Res. 1983; 11(2):369-85. PMC: 325720. DOI: 10.1093/nar/11.2.369. View

Vieira J, Messing J . The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. Gene. 1982; 19(3):259-68. DOI: 10.1016/0378-1119(82)90015-4. View

Depicker A, Stachel S, DHaese P, Zambryski P, Goodman H . Nopaline synthase: transcript mapping and DNA sequence. J Mol Appl Genet. 1982; 1(6):561-73. View

10.

Chilton M, Drummond M, Merio D, Sciaky D, Montoya A, Gordon M . Stable incorporation of plasmid DNA into higher plant cells: the molecular basis of crown gall tumorigenesis. Cell. 1977; 11(2):263-71. DOI: 10.1016/0092-8674(77)90043-5. View

11.

Murai N, Kemp J . Octopine synthase mRNA isolated from sunflower crown gall callus is homologous to the Ti plasmid of Agrobacterium tumefaciens. Proc Natl Acad Sci U S A. 1982; 79(1):86-90. PMC: 345666. DOI: 10.1073/pnas.79.1.86. View

12.

Horsch R, Fraley R, Rogers S, Sanders P, Lloyd A, Hoffmann N . Inheritance of functional foreign genes in plants. Science. 1984; 223(4635):496-8. DOI: 10.1126/science.223.4635.496. View

13.

Braun A, Wood H . Suppression of the neoplastic state with the acquisition of specialized functions in cells, tissues, and organs of crown gall teratomas of tobacco. Proc Natl Acad Sci U S A. 1976; 73(2):496-500. PMC: 335936. DOI: 10.1073/pnas.73.2.496. View

14.

Joos H, Inze D, Caplan A, Sormann M, Van Montagu M, Schell J . Genetic analysis of T-DNA transcripts in nopaline crown galls. Cell. 1983; 32(4):1057-67. DOI: 10.1016/0092-8674(83)90290-8. View

15.

Zambryski P, Holsters M, Kruger K, Depicker A, Schell J, Van Montagu M . Tumor DNA structure in plant cells transformed by A. tumefaciens. Science. 1980; 209(4463):1385-91. DOI: 10.1126/science.6251546. View

16.

Norrander J, Kempe T, Messing J . Construction of improved M13 vectors using oligodeoxynucleotide-directed mutagenesis. Gene. 1983; 26(1):101-6. DOI: 10.1016/0378-1119(83)90040-9. View

17.

Yadav N, Vanderleyden J, Bennett D, Barnes W, Chilton M . Short direct repeats flank the T-DNA on a nopaline Ti plasmid. Proc Natl Acad Sci U S A. 1982; 79(20):6322-6. PMC: 347113. DOI: 10.1073/pnas.79.20.6322. View

18.

Beck E, Ludwig G, Auerswald E, Reiss B, Schaller H . Nucleotide sequence and exact localization of the neomycin phosphotransferase gene from transposon Tn5. Gene. 1982; 19(3):327-36. DOI: 10.1016/0378-1119(82)90023-3. View

19.

Garfinkel D, Simpson R, Ream L, White F, Gordon M, Nester E . Genetic analysis of crown gall: fine structure map of the T-DNA by site-directed mutagenesis. Cell. 1981; 27(1 Pt 2):143-53. DOI: 10.1016/0092-8674(81)90368-8. View