Maize Adh-1 Promoter Sequences Control Anaerobic Regulation: Addition of Upstream Promoter Elements from Constitutive Genes is Necessary for Expression in Tobacco

Overview

Journal EMBO J

Specialties Biotechnology
Molecular Biology

Date 1987 Jan 1

PMID 15981329

Citations 61

Authors

J G Ellis

D J Llewellyn

E S Dennis

W J Peacock

Affiliations

Soon will be listed here.

Abstract

The promoter region of a maize alcohol dehydrogenase gene (Adh-1) was linked to a reporter gene encoding chloramphenicol acetyl transferase (CAT) and transformed stably into tobacco cells using T-DNA vectors. No CAT enzyme activity could be detected in transgenic tobacco plants unless upstream promoter elements from the octopine synthase gene or the cauliflower mosaic virus 35S promoter were supplied in addition to the maize promoter region. CAT enzyme activity and transcription of the chimaeric gene were then readily detected after anaerobic induction. The first 247 bp upstream of the translation initiation codon of the maize Adh-1 gene were sufficient to impose anaerobic regulation on the hybrid gene and S1 nuclease mapping confirmed mRNA initiation is from the normal maize Adh-1 transcription start point.

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References

Lamppa G, Nagy F, Chua N . Light-regulated and organ-specific expression of a wheat Cab gene in transgenic tobacco. Nature. 1985; 316(6030):750-2. DOI: 10.1038/316750a0. View

Ditta G, Stanfield S, Corbin D, Helinski D . Broad host range DNA cloning system for gram-negative bacteria: construction of a gene bank of Rhizobium meliloti. Proc Natl Acad Sci U S A. 1980; 77(12):7347-51. PMC: 350500. DOI: 10.1073/pnas.77.12.7347. View

Otten L, Schilperoort R . A rapid micro scale method for the detection of lysopine and nopaline dehydrogenase activities. Biochim Biophys Acta. 1978; 527(2):497-500. DOI: 10.1016/0005-2744(78)90363-7. View

Hudson G, Farrell P, Barrell B . Two related but differentially expressed potential membrane proteins encoded by the EcoRI Dhet region of Epstein-Barr virus B95-8. J Virol. 1985; 53(2):528-35. PMC: 254667. DOI: 10.1128/JVI.53.2.528-535.1985. View

Herrera-Estrella L, Block M, Messens E, Hernalsteens J, Montagu M, Schell J . Chimeric genes as dominant selectable markers in plant cells. EMBO J. 1983; 2(6):987-95. PMC: 555219. DOI: 10.1002/j.1460-2075.1983.tb01532.x. View

Schwartz M, Sofer W . Alcohol dehydrogenase-negative mutants in Drosophila: defects at the structural locus?. Genetics. 1976; 83(1):125-36. PMC: 1213495. DOI: 10.1093/genetics/83.1.125. View

Otten L, De Greve H, Hernalsteens J, Van Montagu M, Schieder O, Straub J . Mendelian transmission of genes introduced into plants by the Ti plasmids of Agrobacterium tumefaciens. Mol Gen Genet. 1981; 183(2):209-13. DOI: 10.1007/BF00270619. View

An G, Watson B, Stachel S, Gordon M, Nester E . New cloning vehicles for transformation of higher plants. EMBO J. 1985; 4(2):277-84. PMC: 554183. DOI: 10.1002/j.1460-2075.1985.tb03626.x. View

Sachs M, Freeling M, Okimoto R . The anaerobic proteins of maize. Cell. 1980; 20(3):761-7. DOI: 10.1016/0092-8674(80)90322-0. View

10.

Bevan M, Barnes W, Chilton M . Structure and transcription of the nopaline synthase gene region of T-DNA. Nucleic Acids Res. 1983; 11(2):369-85. PMC: 325720. DOI: 10.1093/nar/11.2.369. View

11.

Depicker A, Stachel S, DHaese P, Zambryski P, Goodman H . Nopaline synthase: transcript mapping and DNA sequence. J Mol Appl Genet. 1982; 1(6):561-73. View

12.

Bolivar F . Construction and characterization of new cloning vehicles. III. Derivatives of plasmid pBR322 carrying unique Eco RI sites for selection of Eco RI generated recombinant DNA molecules. Gene. 1978; 4(2):121-36. DOI: 10.1016/0378-1119(78)90025-2. View

13.

Kelley P, Freeling M . Anaerobic expression of maize fructose-1,6-diphosphate aldolase. J Biol Chem. 1984; 259(22):14180-3. View

14.

Franck A, Guilley H, Jonard G, Richards K, Hirth L . Nucleotide sequence of cauliflower mosaic virus DNA. Cell. 1980; 21(1):285-94. DOI: 10.1016/0092-8674(80)90136-1. View

15.

Kelley P, Freeling M . Anaerobic expression of maize glucose phosphate isomerase I. J Biol Chem. 1984; 259(1):673-7. View

16.

Matzke M, Susani M, Binns A, Lewis E, Rubenstein I, Matzke A . Transcription of a zein gene introduced into sunflower using a Ti plasmid vector. EMBO J. 1984; 3(7):1525-31. PMC: 557554. DOI: 10.1002/j.1460-2075.1984.tb02006.x. View

17.

de Vos G, De Beuckeleer M, Van Montagu M, Schell J . Restriction endonuclease mapping of the octopine tumor-inducing plasmid pTiAch5 of Agrobacterium tumefaciens. Plasmid. 1981; 6(2):249-53. DOI: 10.1016/0147-619x(81)90070-6. View

18.

Odell J, Nagy F, Chua N . Identification of DNA sequences required for activity of the cauliflower mosaic virus 35S promoter. Nature. 1985; 313(6005):810-2. DOI: 10.1038/313810a0. View

19.

Jones J, Dunsmuir P, Bedbrook J . High level expression of introduced chimaeric genes in regenerated transformed plants. EMBO J. 1985; 4(10):2411-8. PMC: 554522. DOI: 10.1002/j.1460-2075.1985.tb03949.x. View

20.

Gerlach W, Pryor A, Dennis E, Ferl R, Sachs M, Peacock W . cDNA cloning and induction of the alcohol dehydrogenase gene (Adh1) of maize. Proc Natl Acad Sci U S A. 1982; 79(9):2981-5. PMC: 346332. DOI: 10.1073/pnas.79.9.2981. View