Molecular Features of Community-associated Extended-spectrum-β-lactamase-producing Escherichia Coli Strains in the United States

Overview

Journal Antimicrob Agents Chemother

Publisher American Society for Microbiology

Specialty Pharmacology

Date 2014 Aug 20

PMID 25136013

Citations 3

Authors

Fupin Hu

Jessica A OHara

Jesabel I Rivera

Yohei Doi

Affiliations

Soon will be listed here.

Abstract

We characterized 30 community-associated extended-spectrum-β-lactamase-producing Escherichia coli isolates collected from five hospitals in the United States. Nineteen sequence types were identified. All sequence type 131 (ST131) isolates had the fimH30 allele. IncFII-FIA-FIB was the most common replicon type among the blaCTX-M-carrying plasmids, followed by IncFII-FIA and IncA/C. Restriction analysis of the IncFII-FIA-FIB and IncFII-FIA plasmids yielded related profiles for plasmids originating from different hospitals. The plasmids containing blaCTX-M or blaSHV were stably maintained after serial passages.

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References

Rodriguez-Bano J, Alcala J, Cisneros J, Grill F, Oliver A, Horcajada J . Community infections caused by extended-spectrum beta-lactamase-producing Escherichia coli. Arch Intern Med. 2008; 168(17):1897-902. DOI: 10.1001/archinte.168.17.1897. View

Naseer U, Haldorsen B, Tofteland S, Hegstad K, Scheutz F, Simonsen G . Molecular characterization of CTX-M-15-producing clinical isolates of Escherichia coli reveals the spread of multidrug-resistant ST131 (O25:H4) and ST964 (O102:H6) strains in Norway. APMIS. 2009; 117(7):526-36. DOI: 10.1111/j.1600-0463.2009.02465.x. View

Suzuki S, Shibata N, Yamane K, Wachino J, Ito K, Arakawa Y . Change in the prevalence of extended-spectrum-beta-lactamase-producing Escherichia coli in Japan by clonal spread. J Antimicrob Chemother. 2008; 63(1):72-9. DOI: 10.1093/jac/dkn463. View

Johnson J, Nicolas-Chanoine M, DebRoy C, Castanheira M, Robicsek A, Hansen G . Comparison of Escherichia coli ST131 pulsotypes, by epidemiologic traits, 1967-2009. Emerg Infect Dis. 2012; 18(4):598-607. PMC: 3309662. DOI: 10.3201/eid1804.111627. View

Johnson J, Urban C, Weissman S, Jorgensen J, Lewis 2nd J, Hansen G . Molecular epidemiological analysis of Escherichia coli sequence type ST131 (O25:H4) and blaCTX-M-15 among extended-spectrum-β-lactamase-producing E. coli from the United States, 2000 to 2009. Antimicrob Agents Chemother. 2012; 56(5):2364-70. PMC: 3346636. DOI: 10.1128/AAC.05824-11. View

Park S, Byun J, Choi S, Lee D, Kim S, Kwon J . Molecular epidemiology of extended-spectrum β-lactamase-producing Escherichia coli in the community and hospital in Korea: emergence of ST131 producing CTX-M-15. BMC Infect Dis. 2012; 12:149. PMC: 3458991. DOI: 10.1186/1471-2334-12-149. View

Weissman S, Johnson J, Tchesnokova V, Billig M, Dykhuizen D, Riddell K . High-resolution two-locus clonal typing of extraintestinal pathogenic Escherichia coli. Appl Environ Microbiol. 2012; 78(5):1353-60. PMC: 3294456. DOI: 10.1128/AEM.06663-11. View

Rupp M, Fey P . Extended spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae: considerations for diagnosis, prevention and drug treatment. Drugs. 2003; 63(4):353-65. DOI: 10.2165/00003495-200363040-00002. View

Peirano G, van der Bij A, Gregson D, Pitout J . Molecular epidemiology over an 11-year period (2000 to 2010) of extended-spectrum β-lactamase-producing Escherichia coli causing bacteremia in a centralized Canadian region. J Clin Microbiol. 2011; 50(2):294-9. PMC: 3264152. DOI: 10.1128/JCM.06025-11. View

10.

Colpan A, Johnston B, Porter S, Clabots C, Anway R, Thao L . Escherichia coli sequence type 131 (ST131) subclone H30 as an emergent multidrug-resistant pathogen among US veterans. Clin Infect Dis. 2013; 57(9):1256-65. PMC: 3792724. DOI: 10.1093/cid/cit503. View

11.

van der Bij A, Peirano G, Goessens W, van der Vorm E, van Westreenen M, Pitout J . Clinical and molecular characteristics of extended-spectrum-beta-lactamase-producing Escherichia coli causing bacteremia in the Rotterdam Area, Netherlands. Antimicrob Agents Chemother. 2011; 55(7):3576-8. PMC: 3122469. DOI: 10.1128/AAC.00074-11. View

12.

Carattoli A, Bertini A, Villa L, Falbo V, Hopkins K, Threlfall E . Identification of plasmids by PCR-based replicon typing. J Microbiol Methods. 2005; 63(3):219-28. DOI: 10.1016/j.mimet.2005.03.018. View

13.

Seiffert S, Hilty M, Kronenberg A, Droz S, Perreten V, Endimiani A . Extended-spectrum cephalosporin-resistant Escherichia coli in community, specialized outpatient clinic and hospital settings in Switzerland. J Antimicrob Chemother. 2013; 68(10):2249-54. DOI: 10.1093/jac/dkt208. View

14.

Villa L, Garcia-Fernandez A, Fortini D, Carattoli A . Replicon sequence typing of IncF plasmids carrying virulence and resistance determinants. J Antimicrob Chemother. 2010; 65(12):2518-29. DOI: 10.1093/jac/dkq347. View

15.

Johnson J, Tchesnokova V, Johnston B, Clabots C, Roberts P, Billig M . Abrupt emergence of a single dominant multidrug-resistant strain of Escherichia coli. J Infect Dis. 2013; 207(6):919-28. PMC: 3571447. DOI: 10.1093/infdis/jis933. View

16.

Kang C, Cheong H, Chung D, Peck K, Song J, Oh M . Clinical features and outcome of community-onset bloodstream infections caused by extended-spectrum beta-lactamase-producing Escherichia coli. Eur J Clin Microbiol Infect Dis. 2007; 27(1):85-8. DOI: 10.1007/s10096-007-0401-6. View

17.

Price L, Johnson J, Aziz M, Clabots C, Johnston B, Tchesnokova V . The epidemic of extended-spectrum-β-lactamase-producing Escherichia coli ST131 is driven by a single highly pathogenic subclone, H30-Rx. mBio. 2013; 4(6):e00377-13. PMC: 3870262. DOI: 10.1128/mBio.00377-13. View

18.

Clermont O, Dhanji H, Upton M, Gibreel T, Fox A, Boyd D . Rapid detection of the O25b-ST131 clone of Escherichia coli encompassing the CTX-M-15-producing strains. J Antimicrob Chemother. 2009; 64(2):274-7. DOI: 10.1093/jac/dkp194. View

19.

Minarini L, Gales A, Palazzo I, Darini A . Prevalence of community-occurring extended spectrum beta-lactamase-producing Enterobacteriaceae in Brazil. Curr Microbiol. 2007; 54(5):335-41. DOI: 10.1007/s00284-006-0307-z. View

20.

Doi Y, Park Y, Rivera J, Adams-Haduch J, Hingwe A, Sordillo E . Community-associated extended-spectrum β-lactamase-producing Escherichia coli infection in the United States. Clin Infect Dis. 2012; 56(5):641-8. PMC: 3563390. DOI: 10.1093/cid/cis942. View