Rapid Detection of the O25b-ST131 Clone of Escherichia Coli Encompassing the CTX-M-15-producing Strains

Overview

Journal J Antimicrob Chemother

Publisher Oxford University Press

Specialty Infectious Diseases

Date 2009 May 29

PMID 19474064

Citations 133

Authors

Olivier Clermont

Hiran Dhanji

Mathew Upton

Tarek Gibreel

Andrew Fox

David Boyd

Michael R Mulvey

Patrice Nordmann

Etienne Ruppe

Jean Louis Sarthou

Thierry Frank

Sophie Vimont

Guillaume Arlet

Catherine Branger

Neil Woodford

Erick Denamur

Affiliations

Soon will be listed here.

Abstract

Objectives: Recently, a CTX-M-15 extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli O25b-ST131 clone, belonging to the B2 phylogenetic group and with a high virulence potential, has been reported all over the world, representing a major public health problem. The present study was carried out to develop a rapid and simple detection assay that identifies members of this clone.

Methods: A total of 627 E. coli isolates of which 373 produced an ESBL, collected across four continents, were screened using a O25b-ST131 clone allele-specific PCR for the pabB gene.

Results: One hundred and forty-three ESBL isolates were found positive with the assay. These isolates were all of O25b type and, when studied by multilocus sequence typing (25 cases), were all of ST131. The O25b-ST131 clone was found to produce ESBLs other than CTX-M-15, specifically CTX-M-2, -3, -14, -27, -32 and -61 as well as TEM-24. This clone represents 3% of non-ESBL B2 isolates originating from urinary tract infections in Paris.

Conclusions: We have developed a PCR-based assay that easily identifies a clone with high likelihood of producing ESBLs, including CTX-M-15.

Citing Articles

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Anusic M, Marijan T, Mlinaric Dzepina A, Ticic V, Grskovic L, Vranes J Antibiotics (Basel). 2025; 14(2).

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Extended-Spectrum Beta-Lactamase- and Plasmidic AmpC-Producing among the Faecal Samples in the Bulgarian Community.

Stankova P, Boyanova L, Atanasova D, Mihaylova S, Sredkova M, Gergova R Microorganisms. 2024; 12(9).

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Molecular characterization of extended-spectrum β-lactamase-producing Escherichia coli isolated from lower respiratory tract samples between 2002 and 2019 in the Central Slovenia region.

Hrovat K, Molan K, Seme K, Ambrozic Avgustin J Ann Clin Microbiol Antimicrob. 2024; 23(1):6.

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The clinical, genomic, and microbiological profile of invasive multi-drug resistant in a major teaching hospital in the United Kingdom.

Hamilton W, Coscione S, Maes M, Warne B, Pike L, Khokhar F Microb Genom. 2023; 9(10).

PMID: 37902454 PMC: 10634454. DOI: 10.1099/mgen.0.001122.