Chlamydia Muridarum Infection Associated Host MicroRNAs in the Murine Genital Tract and Contribution to Generation of Host Immune Response

Overview

Journal Am J Reprod Immunol

Specialties Allergy & Immunology
Reproductive Medicine

Date 2014 Jul 1

PMID 24976530

Citations 19

Authors

Rishein Gupta

Tanvi Arkatkar

Jieh-Juen Yu

Shradha Wali

William E Haskins

James P Chambers

Ashlesh K Murthy

Sazaly Abu Bakar

M Neal Guentzel

Bernard P Arulanandam

Affiliations

Soon will be listed here.

Abstract

Problem: Chlamydia trachomatis (CT) is the leading sexually transmitted bacterial infection in humans and is associated with reproductive tract damage. However, little is known about the involvement and regulation of microRNAs (miRs) in genital CT.

Methods: We analyzed miRs in the genital tract (GT) following C. muridarum (murine strain of CT) challenge of wild type (WT) and CD4(+) T-cell deficient (CD4(-/-)) C57BL/6 mice at days 6 and 12 post-challenge.

Results: At day 6, miRs significantly downregulated in the lower GT were miR-125b-5p, -16, -214, -23b, -135a, -182, -183, -30c, and -30e while -146 and -451 were significantly upregulated, profiles not exhibited at day 12 post-bacterial challenge. Significant differences in miR-125b-5p (+5.06-fold change), -135a (+4.9), -183 (+7.9), and -182 (+3.2) were observed in C. muridarum-infected CD4(-/-) compared to WT mice. In silico prediction and mass spectrometry revealed regulation of miR-135a and -182 and associated proteins, that is, heat-shock protein B1 and alpha-2HS-glycoprotein.

Conclusion: This study provides evidence on regulation of miRs following genital chlamydial infection suggesting a role in pathogenesis and host immunity.

Citing Articles

Role of microRNAs in immune regulation and pathogenesis of Chlamydia trachomatis and Chlamydia muridarum infections: a rapid review.

Meewes C, Gupta K, Geisler W Microbes Infect. 2024; 26(8):105397.

PMID: 39025257 PMC: 11609027. DOI: 10.1016/j.micinf.2024.105397.

Normospermic Patients Infected With Role of Dysregulated miR-122-5p, miR-34c-5, and miR-141-3p.

Galdiero M, Trotta C, Schettino M, Cirillo L, Sasso F, Petrillo F Pathog Immun. 2024; 8(2):16-36.

PMID: 38223489 PMC: 10783813. DOI: 10.20411/pai.v8i2.603.

Influence of Fetuin-A on Pulmonary Infection.

Mahjabeen F, Yu J, Chambers J, Gupta R, Arulanandam B Int J Microbiol. 2022; 2022:6082140.

PMID: 35498397 PMC: 9042633. DOI: 10.1155/2022/6082140.

MiR-378b Modulates -Induced Upper Genital Tract Pathology.

Lundy S, Abney K, Ellerson D, Igietseme J, Carroll D, Eko F Pathogens. 2021; 10(5).

PMID: 34067003 PMC: 8151610. DOI: 10.3390/pathogens10050566.

Modulation of Immune Response to by Host miR-135a.

Keck J, Chambers J, Yu J, Cheng X, Christenson L, Guentzel M Front Cell Infect Microbiol. 2021; 11:638058.

PMID: 33928045 PMC: 8076868. DOI: 10.3389/fcimb.2021.638058.

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