Large-scale De Novo DNA Synthesis: Technologies and Applications

Overview

Journal Nat Methods

Specialties Biomedical Engineering
Pathology

Date 2014 May 1

PMID 24781323

Citations 284

Authors

Sriram Kosuri

George M Church

Affiliations

Soon will be listed here.

Abstract

For over 60 years, the synthetic production of new DNA sequences has helped researchers understand and engineer biology. Here we summarize methods and caveats for the de novo synthesis of DNA, with particular emphasis on recent technologies that allow for large-scale and low-cost production. In addition, we discuss emerging applications enabled by large-scale de novo DNA constructs, as well as the challenges and opportunities that lie ahead.

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