A Regulatory Network Governing Gata1 and Gata2 Gene Transcription Orchestrates Erythroid Lineage Differentiation

Overview

Journal Int J Hematol

Specialty Hematology

Date 2014 Mar 19

PMID 24638828

Citations 46

Authors

Takashi Moriguchi

Masayuki Yamamoto

Affiliations

Soon will be listed here.

Abstract

GATA transcription factor family members GATA1 and GATA2 play crucial roles in the regulation of lineage-restricted genes during erythroid differentiation. GATA1 is indispensable for survival and terminal differentiation of erythroid, megakaryocytic and eosinophilic progenitors, whereas GATA2 regulates proliferation and maintenance of hematopoietic stem and progenitor cells. Expression levels of GATA1 and GATA2 are primarily regulated at the transcriptional level through auto- and reciprocal regulatory networks formed by these GATA factors. The dynamic and strictly controlled change of expression from GATA2 to GATA1 during erythropoiesis has been referred to as GATA factor switching, which plays a crucial role in erythropoiesis. The regulatory network comprising GATA1 and GATA2 gives rise to the stage-specific changes in Gata1 and Gata2 gene expression during erythroid differentiation, which ensures specific expression of early and late erythroid genes at each stage. Recent studies have also shed light on the genome-wide binding profiles of GATA1 and GATA2, and the significance of epigenetic modification of Gata1 gene during erythroid differentiation. This review summarizes the current understanding of network regulation underlying stage-dependent Gata1 and Gata2 gene expressions and the functional contribution of these GATA factors in erythroid differentiation.

Citing Articles

Foxi2 and Sox3 are master regulators controlling ectoderm germ layer specification.

Hendrickson C, Blitz I, Hussein A, Paraiso K, Cho J, Klymkowsky M bioRxiv. 2025; .

PMID: 39829826 PMC: 11741269. DOI: 10.1101/2025.01.09.632114.

GATA2 participates in protection against hypoxia-induced pulmonary vascular remodeling.

Shirota Y, Ohmori S, Engel J, Moriguchi T PLoS One. 2024; 19(12):e0315446.

PMID: 39739870 PMC: 11687799. DOI: 10.1371/journal.pone.0315446.

Nagel S, Meyer C, Pommerenke C PLoS One. 2024; 19(12):e0315196.

PMID: 39689089 PMC: 11651569. DOI: 10.1371/journal.pone.0315196.

GATA1 transcription factor targets the gene expression of B19 virus in HEK293 cell line.

Atashi A, Jafaripour L, Froughi K, Behzadifard M Ann Med Surg (Lond). 2024; 86(12):7120-7124.

PMID: 39649852 PMC: 11623887. DOI: 10.1097/MS9.0000000000002244.

A system-level model reveals that transcriptional stochasticity is required for hematopoietic stem cell differentiation.

Herrera J, Bensussen A, Garcia-Gomez M, Garay-Arroyo A, Alvarez-Buylla E NPJ Syst Biol Appl. 2024; 10(1):145.

PMID: 39639033 PMC: 11621455. DOI: 10.1038/s41540-024-00469-8.

References

Takahashi S, Shimizu R, Suwabe N, Kuroha T, Yoh K, Ohta J . GATA factor transgenes under GATA-1 locus control rescue germline GATA-1 mutant deficiencies. Blood. 2000; 96(3):910-6. View

Yomogida K, Ohtani H, Harigae H, Ito E, Nishimune Y, Engel J . Developmental stage- and spermatogenic cycle-specific expression of transcription factor GATA-1 in mouse Sertoli cells. Development. 1994; 120(7):1759-66. DOI: 10.1242/dev.120.7.1759. View

Kaneko H, Shimizu R, Yamamoto M . GATA factor switching during erythroid differentiation. Curr Opin Hematol. 2010; 17(3):163-8. DOI: 10.1097/MOH.0b013e32833800b8. View

Kobayashi E, Shimizu R, Kikuchi Y, Takahashi S, Yamamoto M . Loss of the Gata1 gene IE exon leads to variant transcript expression and the production of a GATA1 protein lacking the N-terminal domain. J Biol Chem. 2009; 285(1):773-83. PMC: 2804226. DOI: 10.1074/jbc.M109.030726. View

McDevitt M, Shivdasani R, Fujiwara Y, Yang H, Orkin S . A "knockdown" mutation created by cis-element gene targeting reveals the dependence of erythroid cell maturation on the level of transcription factor GATA-1. Proc Natl Acad Sci U S A. 1997; 94(13):6781-5. PMC: 21235. DOI: 10.1073/pnas.94.13.6781. View

Suzuki M, Shimizu R, Yamamoto M . Transcriptional regulation by GATA1 and GATA2 during erythropoiesis. Int J Hematol. 2011; 93(2):150-155. DOI: 10.1007/s12185-011-0770-6. View

Chae J, Kim C . CP2 binding to the promoter is essential for the enhanced transcription of globin genes in erythroid cells. Mol Cells. 2003; 15(1):40-7. View

Mukai H, Suzuki M, Nagano M, Ohmori S, Otsuki A, Tsuchida K . Establishment of erythroleukemic GAK14 cells and characterization of GATA1 N-terminal domain. Genes Cells. 2013; 18(10):886-98. DOI: 10.1111/gtc.12084. View

Minegishi N, Suzuki N, Yokomizo T, Pan X, Fujimoto T, Takahashi S . Expression and domain-specific function of GATA-2 during differentiation of the hematopoietic precursor cells in midgestation mouse embryos. Blood. 2003; 102(3):896-905. DOI: 10.1182/blood-2002-12-3809. View

10.

Ferreira R, Ohneda K, Yamamoto M, Philipsen S . GATA1 function, a paradigm for transcription factors in hematopoiesis. Mol Cell Biol. 2005; 25(4):1215-27. PMC: 548021. DOI: 10.1128/MCB.25.4.1215-1227.2005. View

11.

Minegishi N, Ohta J, Suwabe N, Nakauchi H, Ishihara H, Hayashi N . Alternative promoters regulate transcription of the mouse GATA-2 gene. J Biol Chem. 1998; 273(6):3625-34. DOI: 10.1074/jbc.273.6.3625. View

12.

Shimizu R, Ohneda K, Engel J, Trainor C, Yamamoto M . Transgenic rescue of GATA-1-deficient mice with GATA-1 lacking a FOG-1 association site phenocopies patients with X-linked thrombocytopenia. Blood. 2003; 103(7):2560-7. DOI: 10.1182/blood-2003-07-2514. View

13.

Ko L, Engel J . DNA-binding specificities of the GATA transcription factor family. Mol Cell Biol. 1993; 13(7):4011-22. PMC: 359950. DOI: 10.1128/mcb.13.7.4011-4022.1993. View

14.

Yamamoto M, Takahashi S, Onodera K, Muraosa Y, Engel J . Upstream and downstream of erythroid transcription factor GATA-1. Genes Cells. 1997; 2(2):107-15. DOI: 10.1046/j.1365-2443.1997.1080305.x. View

15.

Ohneda K, Yamamoto M . Roles of hematopoietic transcription factors GATA-1 and GATA-2 in the development of red blood cell lineage. Acta Haematol. 2002; 108(4):237-45. DOI: 10.1159/000065660. View

16.

Martin D, Orkin S . Transcriptional activation and DNA binding by the erythroid factor GF-1/NF-E1/Eryf 1. Genes Dev. 1990; 4(11):1886-98. DOI: 10.1101/gad.4.11.1886. View

17.

Iwasaki H, Mizuno S, Wells R, Cantor A, Watanabe S, Akashi K . GATA-1 converts lymphoid and myelomonocytic progenitors into the megakaryocyte/erythrocyte lineages. Immunity. 2003; 19(3):451-62. DOI: 10.1016/s1074-7613(03)00242-5. View

18.

Togarrati P, Suknuntha K . Generation of mature hematopoietic cells from human pluripotent stem cells. Int J Hematol. 2012; 95(6):617-23. DOI: 10.1007/s12185-012-1094-x. View

19.

Whyatt D, Lindeboom F, Karis A, Ferreira R, Milot E, Hendriks R . An intrinsic but cell-nonautonomous defect in GATA-1-overexpressing mouse erythroid cells. Nature. 2000; 406(6795):519-24. DOI: 10.1038/35020086. View

20.

Suzuki N, Suwabe N, Ohneda O, Obara N, Imagawa S, Pan X . Identification and characterization of 2 types of erythroid progenitors that express GATA-1 at distinct levels. Blood. 2003; 102(10):3575-83. DOI: 10.1182/blood-2003-04-1154. View