Lamellar Gel (lβ) Phases of Ternary Lipid Composition Containing Ceramide and Cholesterol

Overview

Journal Biophys J

Publisher Cell Press

Specialty Biophysics

Date 2014 Feb 11

PMID 24507602

Citations 14

Authors

Jon V Busto

Aritz B Garcia-Arribas

Jesus Sot

Alejandro Torrecillas

Juan C Gomez-Fernandez

Felix M Goni

Alicia Alonso

Affiliations

Soon will be listed here.

Abstract

Lipid lateral segregation into specific domains in cellular membranes is associated with cell signaling and metabolic regulation. This phenomenon partially arises as a consequence of the very distinct bilayer-associated lipid physico-chemical properties that give rise to defined phase states at a given temperature. Until now lamellar gel (Lβ) phases have been described in detail in single or two-lipid systems. Using x-ray scattering, differential scanning calorimetry, confocal fluorescence microscopy, and atomic force microscopy, we have characterized phases of ternary lipid compositions in the presence of saturated phospholipids, cholesterol, and palmitoyl ceramide mixtures. These phases stabilized by direct cholesterol-ceramide interaction can exist either with palmitoyl sphingomyelin or with dipalmitoyl phosphatidylcholine and present intermediate properties between raft-associated phospholipid-cholesterol liquid-ordered and phospholipid-ceramide Lβ phases. The present data provide novel, to our knowledge, evidence of a chemically defined, multicomponent lipid system that could cooperate in building heterogeneous segregated platforms in cell membranes.

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