Phase Heterogeneity in Cholesterol-Containing Ternary Phospholipid Lamellar Phases

Overview

Journal ACS Omega

Publisher American Chemical Society

Specialty Chemistry

Date 2023 Feb 27

PMID 36844553

Authors

Deborah L Gater

Keontre I Hughes

Vivian Stojanoff

Abdel F Isakovic

Affiliations

Soon will be listed here.

Abstract

Pseudo-ternary mixtures of lamellar phase phospholipids (DPPC and brain sphingomyelin with cholesterol) were studied below while comparing the influence of cholesterol content, temperature, and the presence of small quantities of vitamin D binding protein (DBP) or vitamin D receptor (VDR). The measurements, conducted by X-ray diffraction (XRD) and nuclear magnetic resonance (NMR), cover a range of cholesterol concentrations (20% mol. wt to 40% mol. wt.) and physiologically relevant temperature range (294-314 K). In addition to rich intraphase behavior, data and modeling are used to approximate the lipids' headgroup location variations under the abovementioned experimental conditions.

References

Epand R, Bain A, Sayer B, Bach D, Wachtel E . Properties of mixtures of cholesterol with phosphatidylcholine or with phosphatidylserine studied by (13)C magic angle spinning nuclear magnetic resonance. Biophys J. 2002; 83(4):2053-63. PMC: 1302294. DOI: 10.1016/S0006-3495(02)73966-0. View

Harper P, Mannock D, LEWIS R, McElhaney R, Gruner S . X-ray diffraction structures of some phosphatidylethanolamine lamellar and inverted hexagonal phases. Biophys J. 2001; 81(5):2693-706. PMC: 1301736. DOI: 10.1016/S0006-3495(01)75912-7. View

Nagle J, Venable R, Maroclo-Kemmerling E, Tristram-Nagle S, Harper P, Pastor R . Revisiting Volumes of Lipid Components in Bilayers. J Phys Chem B. 2019; 123(12):2697-2709. PMC: 6438730. DOI: 10.1021/acs.jpcb.8b12010. View

Pike J, Meyer M, Lee S, Onal M, Benkusky N . The vitamin D receptor: contemporary genomic approaches reveal new basic and translational insights. J Clin Invest. 2017; 127(4):1146-1154. PMC: 5373853. DOI: 10.1172/JCI88887. View

Clarke J, Heron A, Seddon J, Law R . The diversity of the liquid ordered (Lo) phase of phosphatidylcholine/cholesterol membranes: a variable temperature multinuclear solid-state NMR and x-ray diffraction study. Biophys J. 2006; 90(7):2383-93. PMC: 1403185. DOI: 10.1529/biophysj.104.056499. View

White P, Cooke N . The multifunctional properties and characteristics of vitamin D-binding protein. Trends Endocrinol Metab. 2000; 11(8):320-7. DOI: 10.1016/s1043-2760(00)00317-9. View

Bartoccini E, Marini F, Damaskopoulou E, Lazzarini R, Cataldi S, Cascianelli G . Nuclear lipid microdomains regulate nuclear vitamin D3 uptake and influence embryonic hippocampal cell differentiation. Mol Biol Cell. 2011; 22(17):3022-31. PMC: 3164451. DOI: 10.1091/mbc.E11-03-0196. View

Vist M, Davis J . Phase equilibria of cholesterol/dipalmitoylphosphatidylcholine mixtures: 2H nuclear magnetic resonance and differential scanning calorimetry. Biochemistry. 1990; 29(2):451-64. DOI: 10.1021/bi00454a021. View

Li Y, Feng R, Liu M, Guo Y, Zhang Z . Mechanism by Which Cholesterol Induces Sphingomyelin Conformational Changes at an Air/Water Interface. J Phys Chem B. 2022; 126(29):5481-5489. DOI: 10.1021/acs.jpcb.2c03127. View

10.

Busto J, Garcia-Arribas A, Sot J, Torrecillas A, Gomez-Fernandez J, Goni F . Lamellar gel (lβ) phases of ternary lipid composition containing ceramide and cholesterol. Biophys J. 2014; 106(3):621-30. PMC: 3944645. DOI: 10.1016/j.bpj.2013.12.021. View

11.

Chen L, Yu Z, Quinn P . The partition of cholesterol between ordered and fluid bilayers of phosphatidylcholine: a synchrotron X-ray diffraction study. Biochim Biophys Acta. 2007; 1768(11):2873-81. DOI: 10.1016/j.bbamem.2007.07.023. View

12.

Greenwood A, Tristram-Nagle S, Nagle J . Partial molecular volumes of lipids and cholesterol. Chem Phys Lipids. 2006; 143(1-2):1-10. PMC: 2695672. DOI: 10.1016/j.chemphyslip.2006.04.002. View

13.

Shirota K, Yagi K, Inaba T, Li P, Murata M, Sugita Y . Detection of Sphingomyelin Clusters by Raman Spectroscopy. Biophys J. 2016; 111(5):999-1007. PMC: 5018142. DOI: 10.1016/j.bpj.2016.07.035. View

14.

Norman A . Minireview: vitamin D receptor: new assignments for an already busy receptor. Endocrinology. 2006; 147(12):5542-8. DOI: 10.1210/en.2006-0946. View

15.

West J, Zhu Y, Saem S, Moran-Mirabal J, Hitchcock A . X-ray Absorption Spectroscopy and Spectromicroscopy of Supported Lipid Bilayers. J Phys Chem B. 2017; 121(17):4492-4501. DOI: 10.1021/acs.jpcb.7b02646. View

16.

Guo W, Kurze V, Huber T, Afdhal N, Beyer K, Hamilton J . A solid-state NMR study of phospholipid-cholesterol interactions: sphingomyelin-cholesterol binary systems. Biophys J. 2002; 83(3):1465-78. PMC: 1302245. DOI: 10.1016/S0006-3495(02)73917-9. View

17.

Costello A, Alam T . Investigating the impact of cholesterol on magnetically aligned sphingomyelin/cholesterol multilamellar vesicles using static (31)P NMR. Chem Phys Lipids. 2010; 163(6):506-13. DOI: 10.1016/j.chemphyslip.2010.04.001. View

18.

Tsuchikawa H, Monji M, Umegawa Y, Yasuda T, Peter Slotte J, Murata M . Depth-Dependent Segmental Melting of the Sphingomyelin Alkyl Chain in Lipid Bilayers. Langmuir. 2022; 38(18):5515-5524. DOI: 10.1021/acs.langmuir.2c00092. View

19.

Arsov Z, Quaroni L . Detection of lipid phase coexistence and lipid interactions in sphingomyelin/cholesterol membranes by ATR-FTIR spectroscopy. Biochim Biophys Acta. 2008; 1778(4):880-9. DOI: 10.1016/j.bbamem.2007.12.012. View

20.

Mills T, Toombes G, Tristram-Nagle S, Smilgies D, Feigenson G, Nagle J . Order parameters and areas in fluid-phase oriented lipid membranes using wide angle X-ray scattering. Biophys J. 2008; 95(2):669-81. PMC: 2440429. DOI: 10.1529/biophysj.107.127845. View