TL-DNA from Agrobacterium Rhizogenes Plasmid PRi1855 Reduces the Osmotic Pressure in Transformed Plants Grown in Vitro

Overview

Journal Planta

Specialty Biology

Date 2013 Nov 16

PMID 24233742

Citations 2

Authors

G Ooms

J Atkinson

M E Bossen

R A Leigh

Affiliations

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Abstract

Growth, water content, osmotic pressure and solute content were examined for normal potato (Solanum tuberosum L. cv. Desiree) and a derivative (line D9X8a), which was genetically transformed with TL-DNA from Agrobacterium rhizogenes. Plants were grown (i) in vitro, (ii) in a growth chamber and (iii) in the field. In vitro, the transformed potato plants produced more biomass than the untransformed plants, partly because they had a higher water content. Potassium concentration and osmotic pressure were lower in cell sap extracted from the transformed potato shoots. In some cases the difference was as much as 50%. These differences were less clear, absent or reversed in plants from a growth chamber or from the field. In the field, however, transformed potato senesced early. It is suggested that a cellular basis for these observations may be changes induced by Ri TL-DNA expression products in plant membrane properties.

Citing Articles

Developmental regulation of RI TL-DNA gene expression in roots, shoots and tubers of transformed potato (Solanum tuberosum cv. Desiree).

Ooms G, Twell D, Bossen M, Hoge J, Burrell M Plant Mol Biol. 2013; 6(5):321-30.

PMID: 24307382 DOI: 10.1007/BF00034939.

Genetic transformation of Tylophora indica with Agrobacterium rhizogenes A4: growth and tylophorine productivity in different transformed root clones.

Chaudhuri K, Ghosh B, Tepfer D, Jha S Plant Cell Rep. 2005; 24(1):25-35.

PMID: 15660269 DOI: 10.1007/s00299-004-0904-x.

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