Molecular and Genetic Analysis of the Transferred DNA Regions of the Root-inducing Plasmid of Agrobacterium Rhizogenes

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1985 Oct 1

PMID 4044524

Citations 130

Authors

F F White

B H Taylor

G A Huffman

M P Gordon

E W Nester

Affiliations

Soon will be listed here.

Abstract

The T-DNA regions of the root-inducing (Ri) plasmid pRiA4b of Agrobacterium rhizogenes were characterized. Two regions, designated TL-DNA and TR-DNA, were found to be integrated and stably maintained in the plant genome. The TL-DNA spanned a 15- to 20-kilobase region of pRiA4b and was separated from the TR-DNA region by at least 15 kilobases of nonintegrated plasmid DNA. The TR-DNA region also spanned a 15- to 20-kilobase region of pRiA4b and included a region of homology to the tms morphogenic loci of the tumor-inducing (Ti) plasmid of Agrobacterium tumefaciens. Eighteen deletions and 95 transposon insertions were generated in the T-DNA regions and tested for alterations in virulence. Insertions into four loci in the TL-DNA affected the morphology of root formation of Kalanchoë diagremontiana leaves and stems, but had no visible effects on other host plants. Insertions into two loci (tms-1 and tms-2) in the TR-DNA eliminated virulence symptoms on all plants tested, with the exception of K. diagremontiana stems, where sparse root formation occurred. Complementation experiments with Ri and Ti plasmid T-DNA mutations indicate that the tms genes of the two plasmids serve similar functions and suggest a functional relationship between one or more genes of the TL-DNA and the cytokinin synthesis locus tmr of the Ti plasmid.

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References

Koekman B, Ooms G, Klapwijk P, Schilperoort R . Genetic map of an octopine TI-plasmid. Plasmid. 1979; 2(3):347-57. DOI: 10.1016/0147-619x(79)90018-0. View

Akiyoshi D, Morris R, Hinz R, Mischke B, Kosuge T, Garfinkel D . Cytokinin/auxin balance in crown gall tumors is regulated by specific loci in the T-DNA. Proc Natl Acad Sci U S A. 1983; 80(2):407-11. PMC: 393386. DOI: 10.1073/pnas.80.2.407. View

Ditta G, Stanfield S, Corbin D, Helinski D . Broad host range DNA cloning system for gram-negative bacteria: construction of a gene bank of Rhizobium meliloti. Proc Natl Acad Sci U S A. 1980; 77(12):7347-51. PMC: 350500. DOI: 10.1073/pnas.77.12.7347. View

Huffman G, White F, Gordon M, Nester E . Hairy-root-inducing plasmid: physical map and homology to tumor-inducing plasmids. J Bacteriol. 1984; 157(1):269-76. PMC: 215162. DOI: 10.1128/jb.157.1.269-276.1984. View

White F, Nester E . Relationship of plasmids responsible for hairy root and crown gall tumorigenicity. J Bacteriol. 1980; 144(2):710-20. PMC: 294721. DOI: 10.1128/jb.144.2.710-720.1980. View

Akiyoshi D, Klee H, Amasino R, Nester E, Gordon M . T-DNA of Agrobacterium tumefaciens encodes an enzyme of cytokinin biosynthesis. Proc Natl Acad Sci U S A. 1984; 81(19):5994-8. PMC: 391845. DOI: 10.1073/pnas.81.19.5994. View

Watson M, CARTER G, Shaw C . The right hand copy of the nopaline Ti-plasmid 25 bp repeat is required for tumour formation. Nucleic Acids Res. 1984; 12(15):6031-41. PMC: 320054. DOI: 10.1093/nar/12.15.6031. View

Murray M, Thompson W . Rapid isolation of high molecular weight plant DNA. Nucleic Acids Res. 1980; 8(19):4321-5. PMC: 324241. DOI: 10.1093/nar/8.19.4321. View

Ooms G, Hooykaas P, Moolenaar G, Schilperoort R . Grown gall plant tumors of abnormal morphology, induced by Agrobacterium tumefaciens carrying mutated octopine Ti plasmids; analysis of T-DNA functions. Gene. 1981; 14(1-2):33-50. DOI: 10.1016/0378-1119(81)90146-3. View

10.

Costantino P, Spano L, Pomponi M, Benvenuto E, Ancora G . The T-DNA of Agrobacterium rhizogenes is transmitted through meiosis to the progeny of hairy root plants. J Mol Appl Genet. 1984; 2(5):465-70. View

11.

Holsters M, De Waele D, Depicker A, Messens E, Van Montagu M, Schell J . Transfection and transformation of Agrobacterium tumefaciens. Mol Gen Genet. 1978; 163(2):181-7. DOI: 10.1007/BF00267408. View

12.

Stachel S, An G, Flores C, Nester E . A Tn3 lacZ transposon for the random generation of beta-galactosidase gene fusions: application to the analysis of gene expression in Agrobacterium. EMBO J. 1985; 4(4):891-8. PMC: 554276. DOI: 10.1002/j.1460-2075.1985.tb03715.x. View

13.

Byrne M, Koplow J, David C, Tempe J, Chilton M . Structure of T-DNA in roots transformed by Agrobacterium rhizogenes. J Mol Appl Genet. 1983; 2(2):201-9. View

14.

Vieira J, Messing J . The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. Gene. 1982; 19(3):259-68. DOI: 10.1016/0378-1119(82)90015-4. View

15.

Buchholz W, Thomashow M . Comparison of T-DNA oncogene complements of Agrobacterium tumefaciens tumor-inducing plasmids with limited and wide host ranges. J Bacteriol. 1984; 160(1):319-26. PMC: 214719. DOI: 10.1128/jb.160.1.319-326.1984. View

16.

Leong S, Ditta G, Helinski D . Heme biosynthesis in Rhizobium. Identification of a cloned gene coding for delta-aminolevulinic acid synthetase from Rhizobium meliloti. J Biol Chem. 1982; 257(15):8724-30. View

17.

Southern E . Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975; 98(3):503-17. DOI: 10.1016/s0022-2836(75)80083-0. View

18.

Tepfer D . Transformation of several species of higher plants by Agrobacterium rhizogenes: sexual transmission of the transformed genotype and phenotype. Cell. 1984; 37(3):959-67. DOI: 10.1016/0092-8674(84)90430-6. View

19.

Yanofsky M, Montoya A, Knauf V, Lowe B, Gordon M, Nester E . Limited-host-range plasmid of Agrobacterium tumefaciens: molecular and genetic analyses of transferred DNA. J Bacteriol. 1985; 163(1):341-8. PMC: 219119. DOI: 10.1128/jb.163.1.341-348.1985. View

20.

White F, Nester E . Hairy root: plasmid encodes virulence traits in Agrobacterium rhizogenes. J Bacteriol. 1980; 141(3):1134-41. PMC: 293797. DOI: 10.1128/jb.141.3.1134-1141.1980. View