Genetic Modification of Potato Development Using Ri T-DNA

Overview

Journal Theor Appl Genet

Publisher Springer

Specialty Genetics

Date 2013 Nov 21

PMID 24253018

Citations 32

Authors

G Ooms

A Karp

M M Burrell

D Twell

J Roberts

Affiliations

Soon will be listed here.

Abstract

Forty-two potato plants were regenerated from a hairy-root line obtained after infection of a shoot of Solanum tuberosum cv 'Desiree' with Agrobacterium rhizogenes strain LBA 9402 (pRil855). Transformed plants were uniform and had a distinct phenotype and development compared with untransformed controls. Their growth was vigorous, especially early in their development, their roots were abundant and showed reduced geotropism, their leaves were slightly crinkled and glossy and they produced longer tubers with more frequent, prominent eyes. Cytological examination showed that ten of the forty-two transformed plants had either 47 or 49 chromosomes instead of the normal 48. In two of these aneuploids structural changes were observed.

Citing Articles

Segregation of Genes in Two Generations of Engineered Through Wild Type .

Desmet S, Dhooghe E, De Keyser E, Quataert P, Eeckhaut T, Van Huylenbroeck J Front Plant Sci. 2020; 11:859.

PMID: 32676085 PMC: 7333734. DOI: 10.3389/fpls.2020.00859.

Developmental regulation of RI TL-DNA gene expression in roots, shoots and tubers of transformed potato (Solanum tuberosum cv. Desiree).

Ooms G, Twell D, Bossen M, Hoge J, Burrell M Plant Mol Biol. 2013; 6(5):321-30.

PMID: 24307382 DOI: 10.1007/BF00034939.

Ri-plasmid as a helper for introducing vector DNA into alfalfa plants.

Sukhapinda K, Spivey R, Shahin E Plant Mol Biol. 2013; 8(3):209-16.

PMID: 24301125 DOI: 10.1007/BF00015029.

Assessment of the efficiency of cotransformation of the T-DNA of disarmed binary vectors derived from Agrobacterium tumefaciens and the T-DNA of A. rhizogenes.

Hamill J, Prescott A, Martin C Plant Mol Biol. 2013; 9(6):573-84.

PMID: 24277194 DOI: 10.1007/BF00020534.

Transformation of homozygous diploid potato with an Agrobacterium tumefaciens binary vector system by adventitious shoot regeneration on leaf and stem segments.

Visser R, Jacobsen E, Hesseling-Meinders A, Schans M, Witholt B, Feenstra W Plant Mol Biol. 2013; 12(3):329-37.

PMID: 24272868 DOI: 10.1007/BF00043210.

References

White F, Ghidossi G, Gordon M, Nester E . Tumor induction by Agrobacterium rhizogenes involves the transfer of plasmid DNA to the plant genome. Proc Natl Acad Sci U S A. 1982; 79(10):3193-7. PMC: 346381. DOI: 10.1073/pnas.79.10.3193. View

Chilton M, Drummond M, Merio D, Sciaky D, Montoya A, Gordon M . Stable incorporation of plasmid DNA into higher plant cells: the molecular basis of crown gall tumorigenesis. Cell. 1977; 11(2):263-71. DOI: 10.1016/0092-8674(77)90043-5. View

Turgeon R, Wood H, Braun A . Studies on the recovery of crown gall tumor cells. Proc Natl Acad Sci U S A. 1976; 73(10):3562-4. PMC: 431157. DOI: 10.1073/pnas.73.10.3562. View

Ooms G, Karp A, Roberts J . From tumour to tuber; tumour cell characteristics and chromosome numbers of crown gall-derived tetraploid potato plants (Solanum tuberosum cv. 'Maris Bard'). Theor Appl Genet. 2013; 66(2):169-72. DOI: 10.1007/BF00265193. View

Wullems G, Molendijk L, Ooms G, Schilperoort R . Retention of tumor markers in F1 progeny plants from in vitro induced octopine and nopaline tumor tissues. Cell. 1981; 24(3):719-27. DOI: 10.1016/0092-8674(81)90098-2. View

Ooms G, Hooykaas P, Moolenaar G, Schilperoort R . Grown gall plant tumors of abnormal morphology, induced by Agrobacterium tumefaciens carrying mutated octopine Ti plasmids; analysis of T-DNA functions. Gene. 1981; 14(1-2):33-50. DOI: 10.1016/0378-1119(81)90146-3. View

Akiyoshi D, Morris R, Hinz R, Mischke B, Kosuge T, Garfinkel D . Cytokinin/auxin balance in crown gall tumors is regulated by specific loci in the T-DNA. Proc Natl Acad Sci U S A. 1983; 80(2):407-11. PMC: 393386. DOI: 10.1073/pnas.80.2.407. View

De Block M, Herrera-Estrella L, Van Montagu M, Schell J, Zambryski P . Expression of foreign genes in regenerated plants and in their progeny. EMBO J. 1984; 3(8):1681-9. PMC: 557582. DOI: 10.1002/j.1460-2075.1984.tb02032.x. View

Garfinkel D, Simpson R, Ream L, White F, Gordon M, Nester E . Genetic analysis of crown gall: fine structure map of the T-DNA by site-directed mutagenesis. Cell. 1981; 27(1 Pt 2):143-53. DOI: 10.1016/0092-8674(81)90368-8. View

10.

Larkin P, Scowcroft W . Somaclonal variation - a novel source of variability from cell cultures for plant improvement. Theor Appl Genet. 2013; 60(4):197-214. DOI: 10.1007/BF02342540. View

11.

Pomponi M, Spano L, Sabbadini M, Costantino P . Restriction endonuclease mapping of the root-inducing plasmid of Agrobacterium rhizogenes 1855. Plasmid. 1983; 10(2):119-29. DOI: 10.1016/0147-619x(83)90064-1. View

12.

Ooms G, Lenton J . T-DNA genes to study plant development: precocious tuberisation and enhanced cytokinins in A. tumefaciens transformed potato. Plant Mol Biol. 2013; 5(4):205-12. DOI: 10.1007/BF00020638. View

13.

Shepard J, Bidney D, Shahin E . Potato protoplasts in crop improvement. Science. 1980; 208(4439):17-24. DOI: 10.1126/science.208.4439.17. View

14.

Tepfer D . Transformation of several species of higher plants by Agrobacterium rhizogenes: sexual transmission of the transformed genotype and phenotype. Cell. 1984; 37(3):959-67. DOI: 10.1016/0092-8674(84)90430-6. View

15.

Sree Ramulu K, Dijkhuis P, Roest S . Phenotypic variation and ploidy level of plants regenerated from protoplasts of tetraploid potato (Solanum tuberosum L. cv. 'Bintje'). Theor Appl Genet. 2013; 65(4):329-38. DOI: 10.1007/BF00276573. View

16.

Smith E, Townsend C . A PLANT-TUMOR OF BACTERIAL ORIGIN. Science. 1907; 25(643):671-3. DOI: 10.1126/science.25.643.671. View

17.

Huffman G, White F, Gordon M, Nester E . Hairy-root-inducing plasmid: physical map and homology to tumor-inducing plasmids. J Bacteriol. 1984; 157(1):269-76. PMC: 215162. DOI: 10.1128/jb.157.1.269-276.1984. View

18.

Karp A, Nelson R, Thomas E, Bright S . Chromosome variation in protoplast-derived potato plants. Theor Appl Genet. 2013; 63(3):265-72. DOI: 10.1007/BF00304006. View

19.

Horsch R, Fraley R, Rogers S, Sanders P, Lloyd A, Hoffmann N . Inheritance of functional foreign genes in plants. Science. 1984; 223(4635):496-8. DOI: 10.1126/science.223.4635.496. View

20.

Thomas E, Bright S, Franklin J, Lancaster V, Miflin B, Gibson R . Variation amongst protoplast-derived potato plants (Solatium tuberosum cv. 'Maris Bard'). Theor Appl Genet. 2013; 62(1):65-8. DOI: 10.1007/BF00276285. View