Genetic Identification of Functions of TL-DNA Transcripts in Octopine Crown Galls

Overview

Journal EMBO J

Specialties Biotechnology
Molecular Biology

Date 1982 Jan 1

PMID 16453404

Citations 100

Authors

J Leemans

R Deblaere

L Willmitzer

H De Greve

J P Hernalsteens

M Van Montagu

J Schell

Affiliations

Soon will be listed here.

Abstract

The TL-DNA in octopine crown galls encodes seven transcripts most, if not all, expressed from individual promoters. Site-specific deletions and substitutions in the T-region of the octopine plasmid pTiB6S3 indicate some of the functions of the TL-DNA transcripts. Two of the seven genes are sufficient to allow tumorous growth. T-DNA transfer and oncogenicity are controlled by different and independently acting functions. None of the transcripts of TL-DNA appear to be essential for T-DNA transfer. Four, possibly five, of the TL-DNA transcripts act by suppressing organ development. Shoot and root formation are suppressed by the action of different transcripts.

Citing Articles

Nucleotide sequence of the T-DNA region from theA grobacterium tumefaciens octopine Ti plasmid pTi15955.

Barker R, Idler K, Thompson D, Kemp J Plant Mol Biol. 2013; 2(6):335-50.

PMID: 24318453 DOI: 10.1007/BF01578595.

Non-oncogenic T-region mutants ofAgrobacterium tumefaciens do transfer, T-DNA into plant cells.

Hille J, Wullems G, Schilperoort R Plant Mol Biol. 2013; 2(3):155-63.

PMID: 24318209 DOI: 10.1007/BF01578515.

Site-specific mutagenesis in the TR-DNA region of octopine-type Ti plasmids.

Komro C, DiRita V, Gelvin S, Kemp J Plant Mol Biol. 2013; 4(4):253-63.

PMID: 24310843 DOI: 10.1007/BF02418244.

Independent integration and seed-transmission of the TR-DNA of the octopine Ti plasmid pTi Ach5 in Nicotiana plumbaginifolia.

Czako M, Marton L Plant Mol Biol. 2013; 6(2):101-9.

PMID: 24307226 DOI: 10.1007/BF00027303.

Transformation of plant protoplasts with DNA: cotransformation of non-selected calf thymus carrier DNA and meiotic segregation of transforming DNA sequences.

Peerbolte R, Krens F, Mans R, Floor M, Hoge J, Wullems G Plant Mol Biol. 2013; 5(4):235-46.

PMID: 24306764 DOI: 10.1007/BF00020641.

References

Holsters M, Silva B, Van Vliet F, Genetello C, De Block M, DHaese P . The functional organization of the nopaline A. tumefaciens plasmid pTiC58. Plasmid. 1980; 3(2):212-30. DOI: 10.1016/0147-619x(80)90110-9. View

de Vos G, De Beuckeleer M, Van Montagu M, Schell J . Restriction endonuclease mapping of the octopine tumor-inducing plasmid pTiAch5 of Agrobacterium tumefaciens. Plasmid. 1981; 6(2):249-53. DOI: 10.1016/0147-619x(81)90070-6. View

Engler G, Depicker A, Maenhaut R, Villarroel R, Van Montagu M, Schell J . Physical mapping of DNA base sequence homologies between an octopine and a nopaline Ti plasmid of Agrobacterium tumefaciens. J Mol Biol. 1981; 152(2):183-208. DOI: 10.1016/0022-2836(81)90239-4. View

De Beuckeleer M, Lemmers M, de Vos G, Willmitzer L, Van Montagu M, Schell J . Further insight on the transferred-DNA of octopine crown gall. Mol Gen Genet. 1981; 183(2):283-8. DOI: 10.1007/BF00270630. View

Matzke A, Chilton M . Site-specific insertion of genes into T-DNA of the Agrobacterium tumor-inducing plasmid: an approach to genetic engineering of higher plant cells. J Mol Appl Genet. 1981; 1(1):39-49. View

Lemmers M, De Beuckeleer M, Holsters M, Zambryski P, Depicker A, Hernalsteens J . Internal organization, boundaries and integration of Ti-plasmid DNA in nopaline grown gall tumours. J Mol Biol. 1980; 144(3):353-76. DOI: 10.1016/0022-2836(80)90095-9. View

Thomashow M, Nutter R, Montoya A, Gordon M, Nester E . Integration and organization of Ti plasmid sequences in crown gall tumors. Cell. 1980; 19(3):729-39. DOI: 10.1016/s0092-8674(80)80049-3. View

Thomashow M, Nutter R, Postle K, Chilton M, Blattner F, Powell A . Recombination between higher plant DNA and the Ti plasmid of Agrobacterium tumefaciens. Proc Natl Acad Sci U S A. 1980; 77(11):6448-52. PMC: 350302. DOI: 10.1073/pnas.77.11.6448. View

Rao R, Rogers S . Plasmid pKC7: a vector containing ten restriction endonuclease sites suitable for cloning DNA segments. Gene. 1979; 7(1):79-82. DOI: 10.1016/0378-1119(79)90044-1. View

10.

Van Larebeke N, Engler G, Holsters M, Van den Elsacker S, Zaenen I, Schilperoort R . Large plasmid in Agrobacterium tumefaciens essential for crown gall-inducing ability. Nature. 1974; 252(5479):169-70. DOI: 10.1038/252169a0. View

11.

Schroder J, Hillebrand A, Klipp W, Puhler A . Expression of plant tumor-specific proteins in minicells of Escherichia coli: a fusion protein of lysopine dehydrogenase with chloramphenicol acetyltransferase. Nucleic Acids Res. 1981; 9(20):5187-202. PMC: 327514. DOI: 10.1093/nar/9.20.5187. View

12.

Zambryski P, Holsters M, Kruger K, Depicker A, Schell J, Van Montagu M . Tumor DNA structure in plant cells transformed by A. tumefaciens. Science. 1980; 209(4463):1385-91. DOI: 10.1126/science.6251546. View

13.

Garfinkel D, Simpson R, Ream L, White F, Gordon M, Nester E . Genetic analysis of crown gall: fine structure map of the T-DNA by site-directed mutagenesis. Cell. 1981; 27(1 Pt 2):143-53. DOI: 10.1016/0092-8674(81)90368-8. View

14.

Otten L, De Greve H, Hernalsteens J, Van Montagu M, Schieder O, Straub J . Mendelian transmission of genes introduced into plants by the Ti plasmids of Agrobacterium tumefaciens. Mol Gen Genet. 1981; 183(2):209-13. DOI: 10.1007/BF00270619. View

15.

Leemans J, Shaw C, Deblaere R, De Greve H, Hernalsteens J, Maes M . Site-specific mutagenesis of Agrobacterium Ti plasmids and transfer of genes to plant cells. J Mol Appl Genet. 1981; 1(2):149-64. View

16.

Willmitzer L, Simons G, Schell J . The TL-DNA in octopine crown-gall tumours codes for seven well-defined polyadenylated transcripts. EMBO J. 1982; 1(1):139-46. PMC: 553008. DOI: 10.1002/j.1460-2075.1982.tb01137.x. View

17.

Ooms G, Hooykaas P, Moolenaar G, Schilperoort R . Grown gall plant tumors of abnormal morphology, induced by Agrobacterium tumefaciens carrying mutated octopine Ti plasmids; analysis of T-DNA functions. Gene. 1981; 14(1-2):33-50. DOI: 10.1016/0378-1119(81)90146-3. View