IQGAP1 Protein Regulates Nuclear Localization of β-catenin Via Importin-β5 Protein in Wnt Signaling

Overview

Journal J Biol Chem

Specialty Biochemistry

Date 2013 Nov 8

PMID 24196961

Citations 28

Authors

Toshiyasu Goto

Atsushi Sato

Shungo Adachi

Shun-Ichiro Iemura

Tohru Natsume

Hiroshi Shibuya

Affiliations

Soon will be listed here.

Abstract

In the canonical Wnt signaling pathway, the translocation of β-catenin is important for the activation of target genes in the nucleus. However, the molecular mechanisms underlying its nuclear localization remain unclear. In the present study, we found IQGAP1 to be a regulator of β-catenin function via importin-β5. In Xenopus embryos, depletion of IQGAP1 reduced Wnt-induced nuclear accumulation of β-catenin and expression of Wnt target genes during early embryogenesis. Depletion of endogenous importin-β5 associated with IQGAP1 also reduced expression of Wnt target genes and the nuclear localization of IQGAP1 and β-catenin. Moreover, a small GTPase, Ran1, contributes to the nuclear translocation of β-catenin and the activation of Wnt target genes. These results suggest that IQGAP1 functions as a regulator of translocation of β-catenin in the canonical Wnt signaling pathway.

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