Cartilage Tissue Engineering Identifies Abnormal Human Induced Pluripotent Stem Cells

Overview

Journal Sci Rep

Specialty Science

Date 2013 Jun 14

PMID 23760219

Citations 21

Authors

Akihiro Yamashita

Shiying Liu

Knut Woltjen

Bradley Thomas

Guoliang Meng

Akitsu Hotta

Kazutoshi Takahashi

James Ellis

Shinya Yamanaka

Derrick E Rancourt

Affiliations

Soon will be listed here.

Abstract

Safety is the foremost issue in all human cell therapies, but human induced pluripotent stem cells (iPSCs) currently lack a useful safety indicator. Studies in chimeric mice have demonstrated that certain lines of iPSCs are tumorigenic; however a similar screen has not been developed for human iPSCs. Here, we show that in vitro cartilage tissue engineering is an excellent tool for screening human iPSC lines for tumorigenic potential. Although all human embryonic stem cells (ESCs) and most iPSC lines tested formed cartilage safely, certain human iPSCs displayed a pro-oncogenic state, as indicated by the presence of secretory tumors during cartilage differentiation in vitro. We observed five abnormal iPSC clones amoungst 21 lines derived from five different reprogramming methods using three cellular origins. We conclude that in vitro cartilage tissue engineering is a useful approach to identify abnormal human iPSC lines.

Citing Articles

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