Gold Nanoparticle-based Activatable Probe for Sensing Ultralow Levels of Prostate-specific Antigen

Overview

Journal ACS Nano

Publisher American Chemical Society

Specialty Biotechnology

Date 2013 May 21

PMID 23683064

Citations 26

Authors

Dingbin Liu

Xinglu Huang

Zhantong Wang

Albert Jin

Xiaolian Sun

Lei Zhu

Fu Wang

Ying Ma

Gang Niu

Angela R Hight Walker

Xiaoyuan Chen

Affiliations

Soon will be listed here.

Abstract

It is still in high demand to develop extremely sensitive and accurate clinical tools for biomarkers of interest for early diagnosis and monitoring of diseases. In this report, we present a highly sensitive and compatible gold nanoparticle (AuNP)-based fluorescence-activatable probe for sensing ultralow levels of prostate-specific antigen (PSA) in patient serum samples. The limit of detection of the newly developed probe for PSA was pushed down to 0.032 pg/mL, which is more than 2 orders of magnitude lower than that of the conventional fluorescence probe. The ultrahigh sensitivity of this probe was attributed to the high loading efficiency of the dyes on AuNP surfaces and high fluorescence quenching-unquenching abilities of the dye-AuNP pairs. The efficiency and robustness of this probe were investigated in patient serum samples, demonstrating the great potential of this probe in real-world applications.

Citing Articles

Real-Time and Ultrasensitive Prostate-Specific Antigen Sensing Using Love-Mode Surface Acoustic Wave Immunosensor Based on MoS@CuO-Au Nanocomposites.

Yu Y, Xie H, Zhou T, Zhang H, Lu C, Tao R Sensors (Basel). 2024; 24(23).

PMID: 39686170 PMC: 11644860. DOI: 10.3390/s24237636.

Corrigendum: Nano-Theranostics for the Sensing, Imaging and Therapy of Prostate Cancers.

Calatayud D, Neophytou S, Nicodemou E, Giuffrida S, Ge H, Pascu S Front Chem. 2022; 10:919505.

PMID: 35646818 PMC: 9131247. DOI: 10.3389/fchem.2022.919505.

Gold Nanoparticles Based Optical Biosensors for Cancer Biomarker Proteins: A Review of the Current Practices.

Tai J, Fan S, Ding S, Ren L Front Bioeng Biotechnol. 2022; 10:877193.

PMID: 35557858 PMC: 9089302. DOI: 10.3389/fbioe.2022.877193.

Nano-Theranostics for the Sensing, Imaging and Therapy of Prostate Cancers.

Calatayud D, Neophytou S, Nicodemou E, Giuffrida S, Ge H, Pascu S Front Chem. 2022; 10:830133.

PMID: 35494646 PMC: 9039169. DOI: 10.3389/fchem.2022.830133.

Controlled copper in situ growth-amplified lateral flow sensors for sensitive, reliable, and field-deployable infectious disease diagnostics.

Zhou Y, Chen Y, Liu Y, Fang H, Huang X, Leng Y Biosens Bioelectron. 2020; 171:112753.

PMID: 33120235 PMC: 7575433. DOI: 10.1016/j.bios.2020.112753.

References

Giljohann D, Mirkin C . Drivers of biodiagnostic development. Nature. 2009; 462(7272):461-4. PMC: 3936986. DOI: 10.1038/nature08605. View

Fan C, Wang S, Hong J, Bazan G, Plaxco K, Heeger A . Beyond superquenching: hyper-efficient energy transfer from conjugated polymers to gold nanoparticles. Proc Natl Acad Sci U S A. 2003; 100(11):6297-301. PMC: 164440. DOI: 10.1073/pnas.1132025100. View

Nagrath S, Sequist L, Maheswaran S, Bell D, Irimia D, Ulkus L . Isolation of rare circulating tumour cells in cancer patients by microchip technology. Nature. 2007; 450(7173):1235-9. PMC: 3090667. DOI: 10.1038/nature06385. View

Liu D, Xie Y, Shao H, Jiang X . Using azobenzene-embedded self-assembled monolayers to photochemically control cell adhesion reversibly. Angew Chem Int Ed Engl. 2009; 48(24):4406-8. DOI: 10.1002/anie.200901130. View

Mayilo S, Ehlers B, Wunderlich M, Klar T, Josel H, Heindl D . Competitive homogeneous digoxigenin immunoassay based on fluorescence quenching by gold nanoparticles. Anal Chim Acta. 2009; 646(1-2):119-22. DOI: 10.1016/j.aca.2009.05.023. View

Kumar A, Ye T, Takami T, Yu B, Flatt A, Tour J . Reversible photo-switching of single azobenzene molecules in controlled nanoscale environments. Nano Lett. 2008; 8(6):1644-8. DOI: 10.1021/nl080323+. View

de la Rica R, Stevens M . Plasmonic ELISA for the ultrasensitive detection of disease biomarkers with the naked eye. Nat Nanotechnol. 2012; 7(12):821-4. DOI: 10.1038/nnano.2012.186. View

Griffin J, Singh A, Senapati D, Rhodes P, Mitchell K, Robinson B . Size- and distance-dependent nanoparticle surface-energy transfer (NSET) method for selective sensing of hepatitis C virus RNA. Chemistry. 2008; 15(2):342-51. DOI: 10.1002/chem.200801812. View

Perfezou M, Turner A, Merkoci A . Cancer detection using nanoparticle-based sensors. Chem Soc Rev. 2011; 41(7):2606-22. DOI: 10.1039/c1cs15134g. View

10.

Hu M, Yan J, He Y, Lu H, Weng L, Song S . Ultrasensitive, multiplexed detection of cancer biomarkers directly in serum by using a quantum dot-based microfluidic protein chip. ACS Nano. 2010; 4(1):488-94. DOI: 10.1021/nn901404h. View

11.

De M, Rana S, Akpinar H, Miranda O, Arvizo R, Bunz U . Sensing of proteins in human serum using conjugates of nanoparticles and green fluorescent protein. Nat Chem. 2010; 1(6):461-5. PMC: 2782604. DOI: 10.1038/nchem.334. View

12.

Kwong G, von Maltzahn G, Murugappan G, Abudayyeh O, Mo S, Papayannopoulos I . Mass-encoded synthetic biomarkers for multiplexed urinary monitoring of disease. Nat Biotechnol. 2012; 31(1):63-70. PMC: 3542405. DOI: 10.1038/nbt.2464. View

13.

Tabakman S, Lau L, Robinson J, Price J, Sherlock S, Wang H . Plasmonic substrates for multiplexed protein microarrays with femtomolar sensitivity and broad dynamic range. Nat Commun. 2011; 2:466. PMC: 3402035. DOI: 10.1038/ncomms1477. View

14.

Huang C, George S, Lu M, Chaudhery V, Tan R, Zangar R . Application of photonic crystal enhanced fluorescence to cancer biomarker microarrays. Anal Chem. 2011; 83(4):1425-30. PMC: 3039034. DOI: 10.1021/ac102989n. View

15.

Stadler A, Delos Santos J, Stensrud E, Dembska A, Silva G, Liu S . Fluorescent DNA nanotags featuring covalently attached intercalating dyes: synthesis, antibody conjugation, and intracellular imaging. Bioconjug Chem. 2011; 22(8):1491-502. PMC: 3163149. DOI: 10.1021/bc100485f. View

16.

Liu D, Chen W, Sun K, Deng K, Zhang W, Wang Z . Resettable, multi-readout logic gates based on controllably reversible aggregation of gold nanoparticles. Angew Chem Int Ed Engl. 2011; 50(18):4103-7. DOI: 10.1002/anie.201008198. View

17.

Hanash S, Baik C, Kallioniemi O . Emerging molecular biomarkers--blood-based strategies to detect and monitor cancer. Nat Rev Clin Oncol. 2011; 8(3):142-50. DOI: 10.1038/nrclinonc.2010.220. View

18.

Oh E, Hong M, Lee D, Nam S, Yoon H, Kim H . Inhibition assay of biomolecules based on fluorescence resonance energy transfer (FRET) between quantum dots and gold nanoparticles. J Am Chem Soc. 2005; 127(10):3270-1. DOI: 10.1021/ja0433323. View

19.

Nam J, Thaxton C, Mirkin C . Nanoparticle-based bio-bar codes for the ultrasensitive detection of proteins. Science. 2003; 301(5641):1884-6. DOI: 10.1126/science.1088755. View

20.

Thaxton C, Elghanian R, Thomas A, Stoeva S, Lee J, Smith N . Nanoparticle-based bio-barcode assay redefines "undetectable" PSA and biochemical recurrence after radical prostatectomy. Proc Natl Acad Sci U S A. 2009; 106(44):18437-42. PMC: 2773980. DOI: 10.1073/pnas.0904719106. View