Acetylation of Histone Deacetylase 1 Regulates NuRD Corepressor Complex Activity

Overview

Journal J Biol Chem

Specialty Biochemistry

Date 2012 Sep 28

PMID 23014989

Citations 21

Authors

Tao Yang

Wei Jian

Yi Luo

Xueqi Fu

Constance Noguchi

Jorg Bungert

Suming Huang

Yi Qiu

Affiliations

Soon will be listed here.

Abstract

Background: HDAC1-containing NuRD complex is required for GATA-1-mediated repression and activation.

Results: GATA-1 associated with acetylated HDAC1-containing NuRD complex, which has no deacetylase activity, for gene activation.

Conclusion: Acetylated HDAC1 converts NuRD complex from a repressor to an activator during GATA-1-directed erythroid differentiation program.

Significance: HDAC1 acetylation may function as a master regulator for the activity of HDAC1 containing complexes. Histone deacetylases (HDACs) play important roles in regulating cell proliferation and differentiation. The HDAC1-containing NuRD complex is generally considered as a corepressor complex and is required for GATA-1-mediated repression. However, recent studies also show that the NuRD complex is involved in GATA-1-mediated gene activation. We tested whether the GATA-1-associated NuRD complex loses its deacetylase activity and commits the GATA-1 complex to become an activator during erythropoiesis. We found that GATA-1-associated deacetylase activity gradually decreased upon induction of erythroid differentiation. GATA-1-associated HDAC1 is increasingly acetylated after differentiation. It has been demonstrated earlier that acetylated HDAC1 has no deacetylase activity. Indeed, overexpression of an HDAC1 mutant, which mimics acetylated HDAC1, promotes GATA-1-mediated transcription and erythroid differentiation. Furthermore, during erythroid differentiation, acetylated HDAC1 recruitment is increased at GATA-1-activated genes, whereas it is significantly decreased at GATA-1-repressed genes. Interestingly, deacetylase activity is not required for Mi2 remodeling activity, suggesting that remodeling activity may be required for both activation and repression. Thus, our data suggest that NuRD can function as a coactivator or repressor and that acetylated HDAC1 converts the NuRD complex from a repressor to an activator during GATA-1-directed erythroid differentiation.

Citing Articles

HDAC6 regulates human erythroid differentiation through modulation of JAK2 signalling.

Vong P, Messaoudi K, Jankovsky N, Gomilla C, Demont Y, Caulier A J Cell Mol Med. 2022; 27(2):174-188.

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Histone Deacetylases Function in the Control of Early Hematopoiesis and Erythropoiesis.

Vong P, Ouled-Haddou H, Garcon L Int J Mol Sci. 2022; 23(17).

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HDAC1 and PRC2 mediate combinatorial control in SPI1/PU.1-dependent gene repression in murine erythroleukaemia.

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HDAC1 is required for GATA-1 transcription activity, global chromatin occupancy and hematopoiesis.

Yan B, Yang J, Kim M, Luo H, Cesari N, Yang T Nucleic Acids Res. 2021; 49(17):9783-9798.

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