SMAD Versus Non-SMAD Signaling is Determined by Lateral Mobility of Bone Morphogenetic Protein (BMP) Receptors

Overview

Journal J Biol Chem

Specialty Biochemistry

Date 2012 Sep 11

PMID 22961979

Citations 32

Authors

Asja Guzman

Monika Zelman-Femiak

Jan H Boergermann

Sandra Paschkowsky

Peter A Kreuzaler

Peter Fratzl

Gregory S Harms

Petra Knaus

Affiliations

Soon will be listed here.

Abstract

Bone (or body) morphogenetic proteins (BMPs) belong to the TGFβ superfamily and are crucial for embryonic patterning and organogenesis as well as for adult tissue homeostasis and repair. Activation of BMP receptors by their ligands leads to induction of several signaling cascades. Using fluorescence recovery after photobleaching, FRET, and single particle tracking microscopy, we demonstrate that BMP receptor type I and II (BMPRI and BMPRII) have distinct lateral mobility properties within the plasma membrane, which is mandatory for their involvement in different signaling pathways. Before ligand binding, BMPRI and a subpopulation of BMPRII exhibit confined motion, reflecting preassembled heteromeric receptor complexes. A second free diffusing BMPRII population only becomes restricted after ligand addition. This paper visualizes time-resolved BMP receptor complex formation and demonstrates that the lateral mobility of BMPRI has a major impact in stabilizing heteromeric BMPRI-BMPRII receptor complexes to differentially stimulate SMAD versus non-SMAD signaling.

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