CircRFWD2 Promotes Osteogenic Differentiation of Human Dental Pulp Stem Cells by Targeting MiR-6817-5p Through BMP-Smad and P38 MAPK Pathway

Overview

Journal Cell Transplant

Specialties Cell Biology
General Surgery

Date 2021 Oct 25

PMID 34693745

Citations 3

Authors

Xinqi Huang

Xuefeng Pan

Bo Zhang

Wei Huang

Xiao Cen

Jun Liu

Zhihe Zhao

Affiliations

Soon will be listed here.

Abstract

Dental pulp stem cells (DPSCs) are one promising cell source of mesenchymal stem cells in bone tissue engineering. However, it remains unknown that the molecules and signaling pathways involved in osteogenesis of DPSCs. Hence, this study investigated the functional roles and underlying mechanisms of circRFWD2 during osteogenesis of DPSCs. Knockdown of circRFWD2 suppressed osteogenesis of DPSCs significantly. Mechanistically, circRFWD2 could crosstalk with miR-6817-5p, which was an inhibitor of DPSCs osteogenesis. MiR-6817-5p functioned as a sponge of BMPR2, which regulated the phosphorylation of Smad5 and p38 to impact osteogenesis activity of DPSCs. Collectively, circRFWD2/miR-6817-5p/BMPR2 axis could regulate DPSCs osteogenesis via BMP-Smad and p38 MAPK pathway, which are novel mechanisms in the osteogenic differentiation of DPSCs and suggest potential therapeutic methods for bone defects regeneration.

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