Cas5d Protein Processes Pre-crRNA and Assembles into a Cascade-like Interference Complex in Subtype I-C/Dvulg CRISPR-Cas System

Overview

Journal Structure

Publisher Cell Press

Specialties Biochemistry
Biotechnology
Cell Biology
Molecular Biology

Date 2012 Jul 31

PMID 22841292

Citations 109

Authors

Ki Hyun Nam

Charles Haitjema

Xueqi Liu

Fran Ding

Hongwei Wang

Matthew P DeLisa

Ailong Ke

Affiliations

Soon will be listed here.

Abstract

Clustered regularly interspaced short palindromic repeats (CRISPRs), together with an operon of CRISPR-associated (Cas) proteins, form an RNA-based prokaryotic immune system against exogenous genetic elements. Cas5 family proteins are found in several type I CRISPR-Cas systems. Here, we report the molecular function of subtype I-C/Dvulg Cas5d from Bacillus halodurans. We show that Cas5d cleaves pre-crRNA into unit length by recognizing both the hairpin structure and the 3' single stranded sequence in the CRISPR repeat region. Cas5d structure reveals a ferredoxin domain-based architecture and a catalytic triad formed by Y46, K116, and H117 residues. We further show that after pre-crRNA processing, Cas5d assembles with crRNA, Csd1, and Csd2 proteins to form a multi-sub-unit interference complex similar to Escherichia coli Cascade (CRISPR-associated complex for antiviral defense) in architecture. Our results suggest that formation of a crRNA-presenting Cascade-like complex is likely a common theme among type I CRISPR subtypes.

Citing Articles

Research Progress on the Mechanism and Application of the Type I CRISPR-Cas System.

Yang P, Zhang S, Hu D, Li X, Guo Y, Guo H Int J Mol Sci. 2024; 25(23).

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Exploiting activation and inactivation mechanisms in type I-C CRISPR-Cas3 for genome-editing applications.

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PMID: 38126782 PMC: 10865793. DOI: 10.1128/mbio.03025-23.

Bacteriophages suppress CRISPR-Cas immunity using RNA-based anti-CRISPRs.

Camara-Wilpert S, Mayo-Munoz D, Russel J, Fagerlund R, Madsen J, Fineran P Nature. 2023; 623(7987):601-607.

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