Sphingosine-1-phosphate Lyase Expression in Embryonic and Adult Murine Tissues

Overview

Journal J Lipid Res

Publisher Elsevier

Specialty Biochemistry

Date 2012 Jul 12

PMID 22781001

Citations 27

Authors

Alexander D Borowsky

Padmavathi Bandhuvula

Ashok Kumar

Yuko Yoshinaga

Mikhail Nefedov

Loren G Fong

Meng Zhang

Brian Baridon

Lisa Dillard

Pieter de Jong

Stephen G Young

David B West

Julie D Saba

Affiliations

Soon will be listed here.

Abstract

Sphingosine-1-phosphate (S1P) is a bioactive sphingolipid involved in immunity, inflammation, angiogenesis, and cancer. S1P lyase (SPL) is the essential enzyme responsible for S1P degradation. SPL augments apoptosis and is down-regulated in cancer. SPL generates a S1P chemical gradient that promotes lymphocyte trafficking and as such is being targeted to treat autoimmune diseases. Despite growing interest in SPL as a disease marker, antioncogene, and pharmacological target, no comprehensive characterization of SPL expression in mammalian tissues has been reported. We investigated SPL expression in developing and adult mouse tissues by generating and characterizing a β-galactosidase-SPL reporter mouse combined with immunohistochemistry, immunoblotting, and enzyme assays. SPL was expressed in thymic and splenic stromal cells, splenocytes, Peyer's Patches, colonic lymphoid aggregates, circulating T and B lymphocytes, granulocytes, and monocytes, with lowest expression in thymocytes. SPL was highly expressed within the CNS, including arachnoid lining cells, spinal cord, choroid plexus, trigeminal nerve ganglion, and specific neurons of the olfactory bulb, cerebral cortex, midbrain, hindbrain, and cerebellum. Expression was detected in brown adipose tissue, female gonads, adrenal cortex, bladder epithelium, Harderian and preputial glands, and hair follicles. This unique expression pattern suggests SPL has many undiscovered physiological functions apart from its role in immunity.

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