An Electrophoretic Karyotype of Aspergillus Niger

Overview

Journal Mol Gen Genet

Specialties Genetics
Molecular Biology

Date 1990 Nov 1

PMID 2277644

Citations 19

Authors

A J Debets

E F Holub

K Swart

H W Van den Broek

C J Bos

Affiliations

Soon will be listed here.

Abstract

An electrophoretic karyotype of Aspergillus niger was obtained using contour-clamped homogeneous electric field (CHEF) gel electrophoresis. Chromosome-sized DNA was separated into four bands. Seven of the eight linkage groups could be correlated with specific chromosomal bands. For this purpose DNA preparations from seven transformant strains of A. niger each carrying the heterologous amdS gene of Aspergillus nidulans on a different chromosome were analysed. Some of the assignments were confirmed with linkage group-specific A. niger probes. The estimated sizes of the A. niger chromosome range from 3.5 to 6.6 Mb, based on gel migration relative to the chromosomes of Schizosaccharomyces pombe strains, Saccharomyces cerevisiae and A. nidulans. The total genome size of A. niger significantly exceeds that of A. nidulans and is estimated to be about 35.5-38.5 Mb. Electrophoretic karyotyping was used to allocate non-mutant rRNA genes and to estimate the number of plasmids integrated in a high copy number transformant.

Citing Articles

Comparative genomics of citric-acid-producing Aspergillus niger ATCC 1015 versus enzyme-producing CBS 513.88.

Andersen M, Salazar M, Schaap P, van de Vondervoort P, Culley D, Thykaer J Genome Res. 2011; 21(6):885-97.

PMID: 21543515 PMC: 3106321. DOI: 10.1101/gr.112169.110.

Regulation of acid phosphatases in an Aspergillus niger pacC disruption strain.

van den Hombergh J, Maccabe A, van de Vondervoort P, Visser J Mol Gen Genet. 1996; 251(5):542-50.

PMID: 8709960 DOI: 10.1007/BF02173643.

Molecular karyotype of the phytopathogenic fungus Sclerotinia sclerotiorum.

Fraissinet-Tachet L, Fevre M Curr Genet. 1996; 29(5):496-501.

PMID: 8625431 DOI: 10.1007/BF02221520.

Transformation of Sordaria macrospora to hygromycin B resistance: characterization of transformants by electrophoretic karyotyping and tetrad analysis.

Walz M, Kuck U Curr Genet. 1995; 29(1):88-95.

PMID: 8595663 DOI: 10.1007/BF00313198.

Glucoamylase overexpression in Aspergillus niger: molecular genetic analysis of strains containing multiple copies of the glaA gene.

Verdoes J, Punt P, Schrickx J, van Verseveld H, Stouthamer A, van den Hondel C Transgenic Res. 1993; 2(2):84-92.

PMID: 8513339 DOI: 10.1007/BF01969381.

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