The Complete Karyotype of Aspergillus Niger: the Use of Introduced Electrophoretic Mobility Variation of Chromosomes for Gene Assignment Studies

Overview

Journal Mol Gen Genet

Specialties Genetics
Molecular Biology

Date 1994 Jul 8

PMID 8041364

Citations 11

Authors

J C Verdoes

M R Calil

P J Punt

F Debets

K Swart

A H Stouthamer

C A van den Hondel

Affiliations

Soon will be listed here.

Abstract

A method is described for unambiguous assignment of cloned genes to Aspergillus niger chromosomes by CHEF gel electrophoresis and Southern analysis. All of the eight linkage groups (LGs), with the exception of LG VII, have previously been assigned to specific chromosomal bands in the electrophoretic karyotype of A. niger. Using a LG VII-specific probe (nicB gene of A. niger) we have shown that LG VII corresponds to a chromosome of about 4.1 Mb. Furthermore, genetic localization of three unassigned genes (glaA, aglA and pepA) in strains in which these genes had been replaced by a selectable marker gene led to a revised karyotype for the chromosomes corresponding to LGs VIII and VI. The revised electrophoretic karyotype reveals only 5 distinct bands. The presence of three pairs of equally sized chromosomes precluded assignment of genes to one specific chromosome in the wild-type strain. However, unambiguous chromosome assignment of cloned genes using CHEF-Southern analysis was demonstrated using a set of A. niger strains with introduced chromosomal size variation. The availability of these tester strains obviates the need to isolate or construct mutant strains for the purpose of chromosome assignment.

Citing Articles

Comparative genomics of citric-acid-producing Aspergillus niger ATCC 1015 versus enzyme-producing CBS 513.88.

Andersen M, Salazar M, Schaap P, van de Vondervoort P, Culley D, Thykaer J Genome Res. 2011; 21(6):885-97.

PMID: 21543515 PMC: 3106321. DOI: 10.1101/gr.112169.110.

Biorefining of wood: combined production of ethanol and xylanase from waste fiber sludge.

Cavka A, Alriksson B, Rose S, van Zyl W, Jonsson L J Ind Microbiol Biotechnol. 2010; 38(8):891-9.

PMID: 20824487 DOI: 10.1007/s10295-010-0856-9.

Identification of a mitotic recombination hotspot on chromosome III of the asexual fungus Aspergillus niger and its possible correlation with [corrected] elevated basal transcription.

van de Vondervoort P, Langeveld S, Visser J, van Peij N, Pel H, Van Den Hondel C Curr Genet. 2007; 52(3-4):107-14.

PMID: 17684745 PMC: 2071955. DOI: 10.1007/s00294-007-0143-0.

Pulsed-field gel electrophoresis.

Maule J Mol Biotechnol. 1998; 9(2):107-26.

PMID: 9658389 DOI: 10.1007/BF02760813.

The faeA genes from Aspergillus niger and Aspergillus tubingensis encode ferulic acid esterases involved in degradation of complex cell wall polysaccharides.

de Vries R, Michelsen B, Poulsen C, Kroon P, van den Heuvel R, Faulds C Appl Environ Microbiol. 1997; 63(12):4638-44.

PMID: 9406381 PMC: 168785. DOI: 10.1128/aem.63.12.4638-4644.1997.

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