Genetic Analysis of AmdS Transformants of Aspergillus Niger and Their Use in Chromosome Mapping

Overview

Journal Mol Gen Genet

Specialties Genetics
Molecular Biology

Date 1990 Jul 1

PMID 2274031

Citations 9

Authors

A J Debets

K Swart

E F Holub

T Goosen

C J Bos

Affiliations

Soon will be listed here.

Abstract

The Aspergillus nidulans gene coding for acetamidase (amdS) was introduced into A. niger by transformation. Twelve Amd+ transformants were analysed genetically. The amdS inserts were located in seven different linkage groups. In each transformant the plasmid was integrated in only a single chromosome. Our (non-transformed) A. niger strains do not grow on acetamide and are more resistant to fluoroacetamide than the transformants. Diploids hemizygous for the amdS insert have the Amd+ phenotype. We exploited the opportunity for two-way selection in A. niger: transformants can be isolated based on the Amd+ phenotype, whereas counter-selection can be performed using resistance to fluoroacetamide. On this basis we studied the phenotypic stability of the heterologous amdS gene in A. niger transformants as well as in diploids. Furthermore, we mapped the plasmid insert of transformant AT1 to the right arm of chromosome VI between pabA1 and cnxA1, providing evidence for a single transformational insert. The results also show that the amdS transformants of A. niger can be used to localize non-selectable recessive markers and that the method meets the prerequisites for efficient mitotic mapping. We suggest the use of amdS transformants for mitotic gene mapping in other fungi.

Citing Articles

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Novel Strategies for Genomic Manipulation of Trichoderma reesei with the Purpose of Strain Engineering.

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A new method for repeated "self-cloning" promoter replacement in Saccharomyces cerevisiae.

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Glucoamylase overexpression in Aspergillus niger: molecular genetic analysis of strains containing multiple copies of the glaA gene.

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Genetic maps of eight linkage groups of Aspergillus niger based on mitotic mapping.

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PMID: 8428383 DOI: 10.1007/BF00336749.

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