Targeted Gene Knockout by Direct Delivery of Zinc-finger Nuclease Proteins

Overview

Journal Nat Methods

Specialties Biomedical Engineering
Pathology

Date 2012 Jul 4

PMID 22751204

Citations 134

Authors

Thomas Gaj

Jing Guo

Yoshio Kato

Shannon J Sirk

Carlos F Barbas 3rd

Affiliations

Soon will be listed here.

Abstract

Zinc-finger nucleases (ZFNs) are versatile reagents that have redefined genome engineering. Realizing the full potential of this technology requires the development of safe and effective methods for delivering ZFNs into cells. We demonstrate the intrinsic cell-penetrating capabilities of the standard ZFN architecture and show that direct delivery of ZFNs as proteins leads to efficient endogenous gene disruption in various mammalian cell types with minimal off-target effects.

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